摘要
目的探讨转移活性因子GDF11和活性氧在转移性口腔癌中的表达。方法随机选取2016年1月—2018年2月该院口腔癌患者56例,随机分为两组:一组有淋巴结转移的口腔癌组(转移性口腔癌组,20例),一组无淋巴结转移的口腔癌组(无转移性口腔癌组,36例)。在足量的10%甲醛中放置两组患者的一部分手术标本,在室温下固定,保证固定液完全覆盖组织,石蜡包埋后进行免疫组织化学实验;在无酶EP管中放置两组患者的另一部分手术标本,然后第一时间向液态氮中转移并储存,直到分析。将总RNA提取出来,对GDF11基因表达水平进行实时荧光定量检测。进行ROS实验,运用DCFH-DA荧光试验对两组患者的培养基活性氧(ROS)基因表达水平进行检测,然后统计分析两组患者的GDF11和活性氧表达。结果转移性口腔癌组患者的GDF11mRNA、ROS相对水平(6.7±1.4)%、(52.5±10.9)%均显著高于无转移性口腔癌组(3.2±1.0)%、(37.1±12.1)%,差异有统计学意义(t=4.303、6.965,P<0.05)。结论GDF11和活性氧在转移性口腔癌中的表达上升,值得临床充分重视。
Objective To investigate the expression of Trans fer growth factor GDF11 and reactive oxygen species in metastatic oral cancer. Methods A total of 56 patients with oral cancer in this hospital from January 2016 to February 2018 were randomly divided into two groups: a group of oral cancer patients with lymph node metastasis (metastasis oral cancer group, 20 patients), one group without lymph nodes metastatic oral cancer group (no metastatic oral cancer group, 36 cases). A portion of the surgical specimens of the two groups of patients were placed in a sufficient amount of 10% formaldehyde, fixed at room temperature to ensure that the fixative completely covered the tissue, and immunohistochemistry was performed after paraffin embedding;two groups of patients were placed in the enzyme-free EP tube. Another part of the surgical specimen was then transferred to liquid nitrogen for the first time and stored until analysis. Total RNA was extracted and real-time fluorescence quantitative detection of GDF11 gene expression level was performed. The ROS experiment was performed, and the expression of reactive oxygen species (ROS) gene in the two groups was detected by DCFH-DA fluorescence test. The expression of GDF11 and reactive oxygen species in the two groups were statistically analyzed. Results The relative levels of GDF11 mRNA and ROS (6.7±1.4)% and (52.5±10.9)% in the patients with metastatic oral cancer were significantly higher than those in the non-metastatic oral cancer group (3.2±1.0)% and (37.1±12.1)%,the difference was statistically significant(t= 4.303, 6.965, P<0.05). Conclusion The expression of GDF11 and reactive oxygen species in metastatic oral cancer is increasing, which is worthy of clinical attention.
作者
孙丽美
SUN Li-mei(Department of Stomatology,People's Hospital of Anqiu City,Anqiu,Shandong Province,262100 China)
出处
《世界复合医学》
2019年第8期1-3,共3页
World Journal of Complex Medicine
基金
潍坊市卫生计生委科研项目（wfwsjs_2018_080）
关键词
GDF11
活性氧
转移性口腔癌
GDF11
Reactive oxygen species
Metastatic oral cancer
