摘要
采用盆栽试验的方法,研究解淀粉芽孢杆菌(BB2)、长枝木霉(TB2)、棘孢曲霉(BAS1)3种微生物菌剂对辣椒疫病的防控效果以及对土壤性状的影响。通过测定病情指数、植株生物量、土壤化学及土壤微生物等指标,研究不同处理下辣椒疫病的发生程度以及相应土壤性状的变化特征。研究结果表明,3种微生物菌剂均有一定的防控效果;辣椒移栽前期,BAS1处理从第13 d开始病情指数增长较快,第19 d后增长逐渐平缓,而BB2、TB2处理则从第25 d起病情指数开始加速增长;移栽后40 d,各处理的防治效果从高到低依次为BB2、TB2、BAS1,其中BB2的防治效果最高,达到了60%以上。土壤微生物数量测定结果显示,3种微生物菌剂处理后,土壤中相应微生物的数量显著高于其他处理,而辣椒疫霉的数量显著低于对照,表明3种微生物在辣椒根际均有较好的定殖作用,且能抑制病原菌的增殖。
The biocontrol effect of Bacillus amyloliquefaciens (BB2), Trichoderma longibrachiatum (TB2), and Aspergillus aculeatus (BAS1) on pepper blight caused by Phytophthora capsici was investigated through pot experiment. By measuring disease index, plant biomass, soil chemical properties and soil microorganism, the occurrence degree and the variation characteristics of corresponding soil properties under different treatments were studied. The pot experiment suggested that all the three microbial inoculants showed disease inhibition effect on pepper blight. In the early stage of pepper transplanting, the disease index increased rapidly from the isth day of BAS1 treatment, and gradually slowed down from the 19th day. However, the disease index increased rapidly from the 25th day of BB2 and TB2 treatments. The BB2 treatment showed the highest preventive efficiency, which reached to 60%, followed by TB2 and on the 40th day after transplanting. The number of corresponding microorganisms in the three microbial agents treatments was significantly higher than that in the other treatments, while the number of Phytophthora capsici was significantly lower than that in the control, indicating that the three microorganisms had good colonization in the rhizosphere of pepper, and could inhibit the proliferation of pathogens.
作者
叶旻硕
俞键烽
马艳
黄有军
YE Min-shuo;YU Jian-feng;MA Yan;HUANG You-jun(School of Forestry and Bio-technology, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China;Institute of Agricultural Resource and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
出处
《江苏农业学报》
CSCD
北大核心
2019年第4期811-817,共7页
Jiangsu Journal of Agricultural Sciences
基金
国家重点研发计划项目(2016YFD0201007)
江苏省重点研发计划项目(现代农业)(BE2017379-1)
关键词
辣椒疫病
微生物菌剂
土壤微生物
定殖
辣椒疫霉
pepper blight
microbial inoculants
soil microorganism
colonization
Phytophthora capsici
