摘要
本试验旨在研究不同氮利用率山羊瘤胃细菌结构与组成的差异。以30只山羊作为试验动物,通过代谢试验,筛选出高氮利用率组(HNU组)和低氮利用率组(LNU组)的山羊个体。屠宰采集HNU组、LNU组每只山羊瘤胃内容物,用高通量测序技术分析比较组间瘤胃细菌结构与组成的差异性,并用实时荧光定量PCR技术对7种蛋白质分解菌进行定量分析和比较。结果表明:1)30只山羊对饲料氮利用率存在明显的个体差异,HNU组和LNU组的平均氮利用率分别为(56.68±5.54)%和(34.25±4.21)%,组间差异极显著(P<0.01)。2)HNU组和LNU组瘤胃微生物的结构在属水平上有明显差异,HNU组普雷沃氏菌属1(Prevotella 1)、解琥珀酸菌属(Succiniclasticum)、p-1088-a5gutgroup的相对丰度显著低于LNU组(P<0.05),毛螺菌科XPB1014肠道群(Lachnospiraceae XPB1014group)、瘤胃球菌属1(Ruminococcus 1)、瘤胃球菌科UCG004(RuminococcaceaeUCG004)、瘤胃杆菌属(Ruminobacter)、FamilyⅩⅢUCG002、厌氧支原体属(Anaeroplasma)的相对丰度显著高于LNU组(P<0.05)。3)相关分析显示宿主氮利用率与Ruminococcus 1、Ruminobacter、Anaeroplasma的相对丰度呈显著正相关(P<0.05)。4)HNU组中溶纤维丁酸弧菌(Butyrivibriofibrisolvens)、产琥珀酸丝状杆菌属(Fibrobacter succinogens)和瘤胃球菌属sp.HUN007(Ruminococcussp.HUN007)数量显著高于LNU组(P<0.05),而栖息瘤胃普雷沃氏菌(Prevotella ruminicola)、牛链球菌(Streptococcus bovis)、溶糊精琥珀酸弧菌(Succinivibrio dextrinisolvens)和黄色瘤胃球菌(Ruminococcus flavefaciens)数量在2组间没有显著差异(P>0.05)。综上所述,不同氮利用率山羊瘤胃细菌的结构与组成存在显著差异。
The objective of this study was to compare the differences in the bacterial structure and composition in rumen of goats with different nitrogen utilization.Thirty goats were chosen as the experimental animals,according to the data of metabolic test,goats were chosen as the high nitrogen utilization(HNU)group and low nitrogen utilization(LNU)group.Rumen contents of each goat in 2 groups were collected after slaughter and the differences of their bacterial structure and composition were analyzed and compared by high-throughput sequencing technique,and 7 proteolytic bacteria were quantitatively analyzed and compared by real-time PCR.The results showed as follows:1)there were obvious individual differences in the nitrogen utilization in 30 goats,the average nitrogen utilization of HNU group and LNU group was(56.68±5.54)%and(34.25±4.21)%,and there was significant difference between groups(P<0.01).2)The differences of rumen microorganisms structure in HNU group and LNU group were different at the genus level.The relative abundances of Prevotella 1,Succiniclasticum,p-1088-a5gutgroup in HNU group were significantly lower than those in LNU group(P<0.05),and the relative abundances of LachnospiraceaeXPB1014group,Ruminococcus 1,RuminococcaceaeUCG004,Ruminobacter,FamilyXⅢUCG002 and Anaeroplasma groups were significantly higher than those in LNU group(P<0.05).3)The further analysis showed that the relative abundance of Ruminococcus 1,Ruminobacter and Anaeroplasma had a positive relationship with the host nitrogen utilization(P<0.05).4)The numbers of Buyrivibrio fibrisolvens,Fibrobacter succinogenes and Ruminococcussp.HUN007 in HNU group were significantly higher than those in LNU group(P<0.05),and the numbers of Prevotella ruminicola,Streptococcus bovis,Succinivibrio dextrinisolvens and Ruminococcus flavefaciens had no differences between two groups(P>0.05).In summary,there are significant differences on the bacterial structure and composition in rumen of goats with different nitrogen utilization.
作者
李琼
王立志
王之盛
薛白
彭全辉
LI Qiong;WANG Lizhi;WANG Zhisheng;XUE Bai;PENG Quanhui(Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education,Animal Nutrition Institute,Sichuan Agricultural University,Chengdu 611130,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2019年第8期3651-3663,共13页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
四川省学术带头人培养项目(03120244)
关键词
山羊
氮利用率
瘤胃细菌
高通量测序
goats
nitrogen utilization
rumen bacterial
high-throughput sequencing
