摘要
以梨网蝽为材料,从其转录组中筛选功能微卫星序列(EST-SSR),并进行SSR位点的信息分析,开发梨网蝽SSR引物,旨在为深入探究梨网蝽的分子结构奠定理论基础。对梨网蝽转录组中 16 449 条基因数据库的数据进行搜索,共找到6 641个SSR位点,分布在4 420条基因数据库中,发生频率(含有SSR位点的基因数据库与基因数据库总数之比)为26.87%。在对其各组分数量分析中,发现梨网蝽转录组中SSR的主要重复类型是单核苷酸重复,占SSR总数的40.41%;其次是三核苷酸重复,占SSR总数的22.76%。本试验共设计80对梨网蝽EST-SSR引物,其中9对引物可以扩增得到预期的条带。
To develop SSR primers for Stephanitis nashi and further explore its molecular structure, Stephanitis nashi was used as the material, and the functional gene, microsatellite (EST-SSR) sequence, was screened from its transcriptome database for analyzing the information of SSR loci. The search for the total data of 16 449 unigenes in the transcriptome of Stephanitis nashi was carried out. A total of 6 641 SSR loci were found and distributed in 4 420 unigenes. The frequency of occurrence (the ratio of unigenes containing SSR loci to total unigenes) was 26.87%. In the quantitative analysis of its components, it was found that the main repeat type of SSR in the transcriptome of Stephanitis nashi was single nucleotide repeats, accounting for 40.41% of the total number, which was followed by trinucleotide repeats, accounting for 22.76% of the total number. A total of 80 pairs of Stephanitis nashi EST-SSR primers were designed in this experiment, and 9 pairs of SSR primers could amplify the target fragments with expected size. The established method will contribute to development of SSR molecular markers based on Stephanitis nashi database in a large scale.
作者
谢瑾燕
周健
黄大庄
XIE Jinyan;ZHOU Jian;HUANG Dazhang(College of Forestry, Hebei Agricultural University, Baoding 071000, China;College of Landscape and Torism, Hebei Agricultural University, Baoding 071000, China)
出处
《林业与生态科学》
2019年第3期280-285,共6页
Forestry and Ecological Sciences
基金
国家林业局948项目(2013-4-75)
关键词
梨网蝽
微卫星
转录组
SSR引物
Stephanitis nashi
microsatellite
transcriptome
SSR primer
