摘要
为探究野生稷山矮牡丹(Paeonia jishanensis)花青素等抗氧化活性物质生物合成的遗传基础,本文采用高通量测序技术Illumina NextSeqTM500对其幼嫩叶片、嫩茎和花芽混合池样本进行转录组测序分析。共获得39450个unigene,其中3563个unigene中有4106个SSR位点。在Swiss Prot数据库中注释到29420个unigene,其中11个与花青素生物合成过程相关,22个与类黄酮生物合成过程相关,15个与异黄酮生物合成过程相关;NR数据库中注释高达100%,有12个与类黄酮生物合成相关,7个与花青素生物合成相关;GO数据库中注释到24291个unigene,分为生物学过程、细胞组分及分子功能等三大类53个功能组,有21个unigene与黄酮、黄酮醇及异黄酮生物合成有关,4个unigene与花青素合成有关;eggNOG数据库中注释到38238个unigene,涉及植物生长发育过程绝大多数生命活动过程;KEGG数据库注释到5709个unigene,分别定位到6大类别42个代谢途径,其中25个unigene与花青素生物合成相关。研究表明,在稷山矮牡丹转录组序列中,共鉴定和发掘出多个涉及类黄酮化合物生物合成、花青素生物合成过程的相关基因序列及SSR位点,为下一步开展相关代谢合成途径研究奠定了基础。
In order to explore the genetic basis for the biosynthesis of anthocyanin antioxidant, the transcriptome of Paeonia jishanensis was sequenced by Illumina NextSeq TM 500 platform. This results showed that 39 450 unigenes were found by assembly and 4 106 SSR were identified in 3 563 unigenes. All of unigenes were annotated via different databases. 29 420 unigenes were annotated in Swiss Prot database,and 11 unigenes was related to the anthocyanin biosynthesis process, 22 unigenes related to flavonoids and 15 unigenes related to isoflavonoid biosynthesis;NR database annotation were highest at 100%, and 7 unigenes related to anthocyanin and 12 unigenes related to flavonoids biosynthesis;24 291 unigenes were annotated in GO database and divided into the process of 53 function groups;38 238 unigenes were annotated in eggNOG database and classified into 26 functional categories, which involve processing and developments of plants;5 709 unigenes were annotated in KEGG database and grouped into 6 functional categories of 42 metabolism pathways, 25 unigenes involved in the biosynthesis of anthocyanin. This study indicated that some genes and SSR loci which had association with biosynthesis of flavonoids and anthocyanins were identifed. It can provide a foundation for the further researching on metabolic pathways of anthocyanins.
作者
黄春国
王炳华
胡桃花
韩晓丽
李文广
李萍
郝兴宇
HUANG Chunguo;WANG Binghua;HU Taohua;HAN Xiaoli;LI Wenguang;LI Ping;HAO Xingyu(Shanxi Agricultural University College of Agriculture, Taigu 030801, China;Shanxi Agricultural University College of Forestry, Taigu 030801, China;Shanxi Province Agricultural Credit Financing Guarantee Co., Ltd, Taiyuan 030001, China;Shuozhou Meteorological Administration of Shanxi Province, Shuozhou 036000, China)
出处
《激光生物学报》
CAS
2019年第4期360-367,共8页
Acta Laser Biology Sinica
基金
国家自然科学基金项目(31601212)
山西省重点研发计划项目(201703D221033-1)
山西省科技攻关项目(20150312006-2)
山西农谷建设科研专项项目(SXNGJSKYZX201705)
关键词
稷山矮牡丹
转录组测序
基因分析
功能注释
Paeonia jishanensis
transcriptome sequencing
gene analysis
function annotation
