摘要
以拟南芥过氧化物酶体生成蛋白突变体pex5和野生型为试验材料,初步研究PEX5对拟南芥根系抗逆性的影响。结果表明,在MS基础培养基中,突变体pex5的根长相较于野生型根长显著变短;同时,拟南芥pex5突变体株高也低于野生型,说明PEX5基因功能缺失影响了拟南芥根系和茎的生长。pex5突变体在添加氯化钠或甘露醇的MS培养基上培养时,萌发和根伸长受到的抑制显著高于野生型,突变体幼苗的SOD和POD活性也显著低于野生型,说明PEX5参与调控拟南芥根系的抗逆性。本研究为解析PEX5在植物抗逆中的调控机制提供了重要参考。
The Arabidopsis peroxisome generate protein mutant pex5 and the wild type was utilized to study the roles of PEX5 in the stress resistance of Arabidopsis roots. The results showed that the root length of pex5 was significantly shorter than that of the wild type in MS medium. At the same time, the height of Arabidopsis pex5 mutant was also lower than that of the wild type, indicating that the deletion of PEX5 affected the root and stem growth of Arabidopsis . When the pex5 was cultured in MS medium with NaCl or mannitol, the inhibition of germination and root elongation was significantly stronger than that of the wild type, and the SOD and POD activity of the mutant seedlings was significantly lower than that of the wild type, which indicated that PEX5 affected the stress resistance of Arabidopsis roots. This study provides an important reference for understanding the regulation mechanism of PEX5 in plant stress resistance.
作者
詹爽
梁绮雯
罗为桂
肖帅
苏益
萧浪涛
ZHAN Shuang;LIANG Qiwen;LUO Weigui;XIAO Shuai;SU Yi;XIAO Langtao(Hunan Provincial Key Laboratory of Phytohormones and Growth Development,Hunan Agricultural University, Changsha 401028, China)
出处
《激光生物学报》
CAS
2019年第4期336-342,共7页
Acta Laser Biology Sinica
基金
国家自然科学基金项目(31570372)
作物种质创制与资源利用国家重点实验室培育基地开放项目(18KFXM06)
关键词
过氧化物酶体
拟南芥
抗逆
peroxisome
Arabidopsis
stress resistance
