摘要
文章利用微卫星标记对珠江口海域19 个黄唇鱼(Bahaba flavolabiata) DNA 样品进行了遗传多样性研究,以期为黄唇鱼的种质鉴定和保育等研究奠定基础。结果显示,29 个微卫星位点的等位基因数(Na)分别为2~7 个,等位基因总数为121 个,平均等位基因数为4.17 个。有效等位基因数(Ne)介于1.060 6~6.056 1,总和为85.205 0。平均观测杂合度(Ho)和平均期望杂合度(He)分别为0.659 3 和0.597 3。多态性信息含量(PIC)介于0.055 5~0.813 7,平均值为0.542 3,其中19 个位点表现出高度多态性。5 个位点显著偏离Hardy-Weinberg 平衡(校正P≤0.036)。基因丰富度(Rs)介于2~6.976,总和为119.638。基因多样性(GD)介于0.059~0.856。这些数据显示黄唇鱼群体遗传多样性处于较高水平。
We examined the genetic diversity of 19 Bahaba flavolabiata samples collected from the Pearl River estuary based on microsatellite markers, so as to provide references for the identification of its germplasm resources and future breeding. The results show that the number of alleles (Na) in each of the 29 microsatellite loci was 2-7, with a total number of 121 alleles. The average number of alleles in each locus was 4.17. The effective number of alleles (Ne) was 1.060 6-6.056 1, with a sum of 85.205 0. The average values of the observed heterozygosity (Ho) and the expected heterozygosity (He) were 0.659 3 and 0.597 3, respectively. The value of polymorphism information content (PIC) was 0.055 5-0.813 7, with an average of 0.542 3, and 19 loci showed high polymorphism. Five loci deviated significantly from the Hardy-Weinberg equilibrium (corrected P≤0.036). The allelic richness (Rs) was 2-6.976, with a sum of 119.638. The genetic diversity (GD) was 0.059.0.856. It is suggested that most of the SSR loci developed in this study are highly polymorphic, and the genetic diversity of B. flavolabiata population is at a higher level.
作者
赵彦花
区又君
温久福
李加儿
周慧
ZHAO Yanhua;OU Youjun;WEN Jiufu;LI Jia’er;ZHOU Hui(Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization,Ministry of Agriculture and Rural Affairs,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China;National Demonstration Center for Experimental Fisheries Science Education,College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China)
出处
《南方水产科学》
CAS
CSCD
北大核心
2019年第4期127-132,共6页
South China Fisheries Science
基金
广州市科技计划项目(201604020088)
广东省省级科技计划项目(2017B020204002)
关键词
黄唇鱼
微卫星标记
遗传多样性
Bahaba flavolabiata
microsatellite makers
genetic diversity
