摘要
为探讨发酵豆粕替代鱼粉对卵形鲳鲹(Trachinotus ovatus)生长和血清生化的影响,该研究以鱼粉(fishmeal,FM)为基础蛋白源配制了5 组饲料[分别添加0%(FM)、25%(FSM25)、50%(FSM50)、75%(FSM75)和100%(FSM100)的发酵豆粕(fermented soybean meal,FSM)替代鱼粉]。为消除限制性氨基酸的影响,分别添加0%、0.10%、0.22%、0.35%和0.45%的赖氨酸及0%、0.14%、0.27%、0.41%和0.54%的蛋氨酸。结果显示,FSM25 和FSM50 组的增重率、特定生长率、肥满度和采食量与FM 组无显著差异(P>0.05),FSM100 组饲料系数显著高于FM 组(P< 0.05)。发酵豆粕各组的肌肉水分、灰分、粗蛋白和粗脂肪含量与FM 组相比无显著差异(P>0.05)。FSM75 和FSM100 组血清中的谷草转氨酶(AST)活性和总蛋白(TP)含量分别显著高于和低于FM 组(P<0.05),FSM100 组谷丙转氨酶(ALT)和超氧化物歧化酶(SOD)活性显著高于FM 组(P<0.05)。FSM75 和FSM100 组肝脏IGF-1 和GH 基因表达量显著低于FM 组(P<0.05)。综上,在添加限制性氨基酸条件下,发酵豆粕替代鱼粉不超过50%不会对卵形鲳鲹产生不利影响,根据回归曲线得出最佳替代水平为17.5%。
To investigate the effects of substitution of fish meal by fermented soybean meal on the growth performance and serum biochemistry of Trachinotus ovatus, we have formulated five experimental diets, adding 0%(FM), 25%(FSM25), 50%(FSM50), 75%(FSM75) and 100%(FSM100) fermented soybean meal to replace fish meal. In order to eliminate the effects of limiting amino acids, we added 0%, 0.10%, 0.22%, 0.35% and 0.45% lysine, as well as 0%, 0.14%, 0.27%, 0.41% and 0.54% methionine, respectively. The results show that the weight again rate, specific gain rate, specific growth rate and feed intake did not have significant difference among FSM25, FSM50 and FM groups (P>0.05), but the feed conversion ratio in FSM100 group was significantly higher than that in FM group (P<0.05). There was insignificant difference in the muscle moisture, ash, crude protein, crude fat contents between the fermented soybean meal groups and FM group (P>0.05). The serum aspartate aminotransferase (AST) activities and total protein (TP) contents in FSM75 and FSM100 groups were significantly higher and lower than those in FM group, respectively (P<0.05). The activities of alanine aminotransferase (ALT) and superoxide dismutase (SOD) in FM100 group were significantly higher than those in FM group, respectively (P<0.05). The relative expressions of IGF-1 and GH genes in FSM75 and FSM100 groups were significantly lower than those in FM group (P<0.05). In summary, by adding limiting amino acids, the substitution of fish meal by fermented soybean meal not exceeding 50% does not have adverse affects on T. ovatus. According to the regression curve, the substitution level of 17.5% will achieve the best effects.
作者
李秀玲
刘宝锁
张楠
郭梁
江世贵
张殿昌
LI Xiuling;LIU Baosuo;ZHANG Nan;GUO Liang;JIANG Shigui;ZHANG Dianchang(Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization,Ministry of Agriculture and Rural Affairs,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China;College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;Guangdong Provincial Engineer Technology Research Center of Marine Biological Seed Industry,Guangzhou 510300,China)
出处
《南方水产科学》
CAS
CSCD
北大核心
2019年第4期68-75,共8页
South China Fisheries Science
基金
广东省科技计划项目(2019B030316030)
国家科技基础条件平台建设运行项目(2018DKA30470)
现代农业产业技术体系建设专项资金(CARS-47-G07)
广东省现代农业产业技术体系创新团队建设专项资金
关键词
卵形鲳鲹
发酵豆粕
生长性能
血清生化
生长相关基因
Trachinotus ovatus
fermented soybean meal
growth performance
serum biochemistry
growth-related genes
