摘要
目的考察淫羊藿苷在不同条件(pH、温度、缓冲液)下的降解动力学行为。方法 HPLC法测定淫羊藿苷含有量后,计算其在不同pH(1.0、3.0、4.5、6.0、6.8、8.0)、温度(4、25、37、60℃)、缓冲液(0.1、0.3、0.5 mol/L磷酸盐、柠檬酸盐、醋酸盐)下的降解动力学参数。结果淫羊藿苷降解符合一级动力学方程,在pH 6.8时降解速率最小,稳定性最强。其降解速率随着温度、磷酸盐浓度升高而增加,随着醋酸盐浓度升高而降低,在不同浓度柠檬酸盐缓冲液中无明显变化。结论淫羊藿苷降解与pH、温度、缓冲液有一定关系。
AIM To investigate the degradation kinetic behaviors of icariin under different conditions ( pH, temperature,buffer). METHODS HPLC was adopted in the content determination of icariin,after which the degradation kinetic parameters under different pH values ( 1. 0,3. 0,4. 5,6. 0,6. 8,8. 0),temperatures ( 4,25, 37,60 ℃) and buffers ( 0. 1、0. 3、0. 5 mol /L phosphates,citrates,acetates) were calculated. RESULTS The degradation of icariin accorded with first-order kinetic equation,whose minimum degradation rate and strongest stability were observable at the pH value of 6. 8. The degradation rate was elevated with the increase of temperature and phosphate concentration,and reduced with the increase of acetate concentration,which demonstrated no obvi- ous changes in different concentrations of citrate buffers. CONCLUSION The degradation of icariin has certain relationships with pH,temperature and buffer.
作者
陶俊烨
丁选胜
吴正红
丁雨寅
宋征宇
王勤
TAO Jun-ye;DING Xuan-sheng;WU Zheng-hong;DING Yu-yin;SONG Zheng-yu;WANG Qin(School of Basic Medicine and Clinical Pharmacy,China Pharmaceutical University,Nanjing 211100,China;School of Pharmacy,China Pharmaceutical University,Nanjing 211100,China)
出处
《中成药》
CAS
CSCD
北大核心
2019年第8期1763-1767,共5页
Chinese Traditional Patent Medicine
基金
国家“重大新药创制”科技重大专项资助项目(2017ZX09301004)
