摘要
【目的】立枯病、红腐病、枯萎病和黄萎病是新疆棉花上的4种主要土传病害,研究4种病害的病原菌在新疆棉花上的侵染动态,分析各自的侵染始期和最佳防治时期,为病害高效防控提供依据。【方法】针对4种主要病害的病原菌,对混合接菌后分期采集的棉株进行特异性引物PCR检测,依据检测结果,进行各病原菌侵染动态分析及混合侵染分析。【结果】4种病原菌从棉花子叶期即能侵染;立枯丝核菌侵染相对较早、较快,拟轮枝镰孢霉次之;2种病原菌均在棉苗前期有较高的侵染株率,3叶期达到高峰,此后侵染株率逐渐降低;而尖孢镰孢霉和大丽轮枝菌,虽从子叶期开始即可侵染,但侵染株率较低,直到蕾铃期侵染株率呈渐增趋势;病原菌之间的混合侵染普遍存在,以两菌混合侵染居多。【结论】子叶期为4种病原菌的侵染初期;立枯丝核菌和拟轮枝镰孢霉以苗期侵染为特点;尖孢镰孢霉和大丽轮枝菌以子叶期到蕾铃期均可侵染为特点;病原菌之间常有混合侵染发生。
【Objective】Rhizoctonia damping off,Fusarium red rot,Fusarium wilt and Verticillium wilt are four major soil-borne diseases on cotton in Xinjiang.This project aims to clarify the infection dynamics of the four diseases of cotton in Xinjiang and analyze the initial infection period and the best control period of each disease in the hope of providing a basis for the high efficiency prevention and control of the disease.【Methods】In this study,specific primer PCR was used to detect the pathogen of four main diseases,and the dynamic analysis and mixed infection analysis of each pathogen were carried out according to the detection results.【Results】The results indicated that the four pathogens could be infested from the cotyledon stage of cotton;the infection of Rhizoctonia solani was detected relatively earlier and faster,followed by Fusarium verticillioides,the infection rate of the two pathogens was high in the early stage of cotton seedling and reached the peak at the three-leaf stage,and the detected rate decreased gradually after that.Although the Fusarium oxysporum and Verticillium dahliae could also be infected from the cotyledon stage,but the rate of detection in the cotyledon to the one-leaf stage was low,Until the time of the boll stage invasion plant rate was gradually increasing.The mixed infection between the pathogens was widely spread,and the mixed infection of the two species pathogens was the most common.【Conclusions】Cotyledon stage is the infection start period of four species pathogens.R.solani and F.verticillioides is characterized by seedling invasion,while F.oxysporum and V.dahliae is characterized by invasion from cotyledon stage to boll stage;There are often mixed infections among pathogens.
作者
许泰百
萨吉达木·艾则孜
吴琼
李玉华
李进
郭庆元
XU Tai-bai;Sajidamu Aizezi;WU Qiong;LI Yu-hua;LI Jin;GUO Qing-yuan(College of Agronomy,Xinjiang Agricultural University,Urumqi 830052,China;Research Institute of Nuclear Technology and Biotechnology,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China)
出处
《新疆农业科学》
CAS
CSCD
北大核心
2019年第5期910-918,共9页
Xinjiang Agricultural Sciences
基金
国家重点研发计划“棉花化肥农药减施技术集成研究与示范”(2017YFD0201903)
公益性行业(农业)科研专项“棉花苗期根腐类病害综合治理技术”(201503112-6)
新疆农业大学研究生科研创新项目“4种棉花苗期病害相互关系影响”研究(XJAUGRI2016-013)~~
关键词
棉花
土传病害
立枯丝核菌
拟轮枝镰孢霉
尖孢镰孢霉
大丽轮枝菌
侵染动态
cotton
soil-borne diseases
Rhizoctonia solani
Fusarium verticillioides
Fusarium oxysporum
Verticillium dahliae
infection dynamics
