摘要
为从薰衣草提取物中寻找潜在的治疗阿尔茨海默病的乙酰胆碱酯酶抑制剂。采用经乙醇提取,聚酰胺柱、HP20树脂柱及制备液相分离纯化后,得到30个薰衣草提取物,测定其多酚含量,并以石杉碱甲为阳性对照,采用酶标仪测定405nm处吸光度值以筛选其乙酰胆碱酯酶抑制活性并计算抑制率,分析二者相关性。结果表明:薰衣草提取物对乙酰胆碱酯酶表现出了不同的抑制作用(抑制率3.00%~82.78%),其中7个提取物作用较为显著,抑制率均大于50%;有4个提取物表现出了微弱的抑制作用(抑制率小于10%);另外,近一半的薰衣草提取物多酚含量大于20μg·mL-1。薰衣草提取物具有乙酰胆碱酯酶抑制活性,表明其含有抑制乙酰胆碱酯酶的成分,经过相关性分析,多酚可能不是起主要作用的活性物质,为进一步跟踪分离活性成分奠定基础。
In order to find potential inhibitors fromLavandula angustifolia Mill.extract for acetylcholin esterase that can be used in the treatment of Alzheimer′s disease.Lavandula angustifolia Mill.was extracted by ethanol and purified by polyamide column,HP20 resin column and preparative liquid chromatography.The Folin-phenol reagent method was used for the determination of total phenolics Lavandula angustifolia Mill.Extract Totally 30 extracts fromLavandula angustifolia Mill.were served as inhibitors,Huperzine A was used as the positive control to detect the absorbance value at 405 nmin vitro enzymatic reaction system by enzymelabeling instrument.The inhibitory activity on the extracts was calculated.The results showed that Lavandula angustifolia Mill.extract showed a certain inhibitory activity on acetylcholin esterase(inhibition rate was3.00%~82.78%).7 Lavandula angustifolia Mill.extracts showed significant inhibition on acetylcholin esterase(inhibition rate was higher than 50%),while 4 Lavandula angustifolia Mill.extracts showed faint inhibition on acetylcholin esterase(inhibition rate was less than 10%).Half of Lavandula angustifolia Mill.extract showed the content of polyphenols was higher than 20μg·mL-1.The most of extract showed a certain inhibitory activity on acetylcholin esterase.Through the correlation analysis,polyphenols may not be the main active substance.
作者
王苗苗
韩飞
严欢
张倩
李慕春
WANG Miao-miao;HAN Fei;YAN Huan;ZHANG Qian;LI Mu-chun(Xinjiang Academy of Analysis and Testing,Urumqi 830011,China)
出处
《黑龙江农业科学》
2019年第8期124-127,共4页
Heilongjiang Agricultural Sciences
基金
新疆地产中药民族药新药研发培育项目(2017-02-07)
关键词
薰衣草提取物
乙酰胆碱酯酶
抑制剂
阿尔茨海默病
酶标仪
Lavandula angustifolia Mill. extract
acetylcholin esterase
inhibitor
Alzheimer’s disease
enzyme-labeling instrument
