摘要
目的:检测lncRNA LINC00886在食管鳞状细胞癌(ESCC)组织及细胞系中的表达及其对Eca109细胞体外增殖、迁移及侵袭的影响。方法:收集2014年6月至2016年12月河北医科大学第四医院生物标本库69例ESCC手术患者的癌及对应的癌旁组织标本,以及ESCC细胞系Eca109、TE13、TE1、Kyse150、Yes-2和Kyse170,用qPCR法检测LINC00886在ESCC组织及细胞系中的表达情况。分别用pIRES2-LINC00886、p IRES2-NC转染Eca109细胞,用qPCR法检测pIRES2-LINC00886转染Eca109细胞后LINC00886的过表达效率;用MTS、克隆形成实验、划痕愈合实验、Transwell侵袭实验分别检测过表达LINC00886对Eca109细胞增殖、迁移及侵袭能力的影响。结果:在ESCC组织中LINC00886表达水平明显低于癌旁组织(P<0.01),其表达水平与肿瘤TNM分期和淋巴结转移相关(均P<0.05)。LINC00886在ESCC细胞中的表达水平也低于对照组(均P<0.01)。转染pIRES2-LINC00886后,Eca109细胞中LINC00886的表达水平显著高于对照组(均P<0.05)。与对照组相比,过表达LINC00886明显抑制Eca109细胞的增殖、迁移和侵袭能力(均P<0.01)。结论:lncRNA LINC00886低表达可能与ESCC的发生发展相关,过表达LINC00886可抑制ESCC细胞的增殖、迁移与侵袭能力。
Objective:To investigate the expression of lncRNA LINC00886 in human esophageal squamous cell carcinoma(ESCC tissues and cell lines,and its effects on proliferation,migration and invasion of Eca109 cells.Methods:The cancer tissues and corresponding para-cancerous tissues of 69 ESCC patients were collected in the biological specimen bank of the Fourth Hospital of Hebei Medical University from June 2014 to December 2016;the ESCC cell lines Eca109,TE13,TE1,Kyse150,Yes-2 and Kyse170 were also collected.LINC00886 gene expression in ESCC tissues and cell lines was detected by qPCR.Eca109 cells were transfected with pIRES2-LINC00886 and pIRES2-NC,respectively,and the overexpression efficiency of LINC00886 gene in Eca109 cells was detected by q PCR;MTS,clone formation assay,wound-healing assay and Transwell invasion assay were respectively used to detect the effect of LINC00886 over-expression on proliferation,migration and invasion ability of Eca109 cells.Results:The expression of LINC00886 gene in ESCC tissues was significantly lower than that in para-cancerous tissues(P<0.01),and its expression level was associated with tumor TNM stage and lymph node metastasis(both P<0.05).The expression level of LINC00886 gene in ESCC cell lines was also lower than that of the control group(all P<0.01).Compared with control group,the expression level of LINC00886 gene was significantly higher in Eca109 cells transfected with p IRES2-LINC00886(both P<0.05).Compared with the control group,LINC00886 overexpression significantly inhibited the proliferation,migration and invasion abilities of Eca109 cells(all P<0.01).Conclusion:The decreased expression of LINC00886 gene may be related to the occurrence and development of ESCC.Over-expression of LINC00886 gene inhibits the proliferation,migration and invasion abilities of ESCC cells.
作者
杨柳
梁佳
沈素朋
刘磊
任利兵
郭炜
董稚明
YANG Liu;LIANG Jia;SHEN Supeng;LIU Lei;REN Libing;GUO Wei;DONG Zhiming(Cancer Institute,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,Hebei,China;Departmentof Thoracic Surgery,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,Hebei,China;Department of ThoracicSurgery,Central Hospital of Handan City,Handan 056001,Hebei,China)
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
北大核心
2019年第7期751-756,共6页
Chinese Journal of Cancer Biotherapy
基金
国家自然科学基金资助项目(No.81572441)~~
