摘要
目的探索不同浓度维生素E对γ射线照射后果蝇幼虫敏感性的影响。方法使用不同浓度(100、500、1000、1500mg/L)维生素E处理W1118果蝇幼虫,^137Csγ射线50Gy进行照射,测定果蝇幼虫的蛹化率和羽化率、羽化后48h死亡率、过氧化氢酶(CAT)活性和谷胱甘肽(GSH)含量,分析其抗氧化能力和氧化损伤状态。组间的比较采用LSD-t检验。结果1000、1500mg/L维生素E组果蝇幼虫的蛹化率、羽化率、攀爬能力、CAT活性、GSH含量均明显比单纯照射对照组高,且差异均有统计学意义(t=2.864~16.462,均P<0.05)。与未照射对照组比较,1000mg/L和1500mg/L维生素E组的果蝇幼虫羽化为成虫48h内的死亡率分别从(54.0±5.0)%降低至(36.1±7.6)%和(37.5±5.8)%,差异均有统计学意义(t=3.386,P=0.028;t=3.718,P=0.021)。结论维生素E通过减少氧化性应激来减缓果蝇幼虫辐照后的氧化损伤,并能提高果蝇的辐射抗性。
Objective To explore the effects of different concentrations of vitamin E on the sensitivity of fruit fly larvae after gamma-ray irradiation. Methods Different concentrations(100, 500, 1000, and 1500 mg/L) of vitamin E were used to treat W1118 fruit fly larvae, and ^137Cs gamma-ray irradiation of 50 Gy was utilized. The pupation and eclosion rates, mortality rate after eclosion for 48 h, catalase activity(CAT), and glutathione(GSH) content of fruit fly larvae were measured, and the antioxidant capacity and oxidative damage status were analyzed. LSD t-test was used to compare groups. Results The pupation rate, emergence rate, climbing ability, CAT activity, and GSH content of fruit fly larvae in the vitamin E(1000 mg/L and 1500 mg/L) group were significantly higher than those in the control group, and the differences were statistically significant(t=2.864-16.462, all P<0.05). In comparison with the non-irradiated control group, the mortality rate of fruit fly larvae in the vitamin E group with concentrations of 1000 mg/L and 1500 mg/L significantly decreased from(54.0±5.0)% to(36.1±7.6)% and(37.5±5.8)%, respectively, within 48 h of emergence to adult(t=3.386, P=0.028;t=3.718, P=0.021). Conclusion Vitamin E can slow down the oxidative damage of Drosophila melanogaster larvae after irradiation by reducing oxidative stress, as well as improve the radiation resistance of Drosophila melanogaster.
作者
贺特
马云
王五洲
贺俊彦
蒋雨薇
曹旭琴
何淑雅
He Te;Ma Yun;Wang Wuzhou;He Junyan;Jiang Yuwei;Cao Xuqing;He Shuya(Department of Biochemistry and Molecular Biology,Hengyang Medical College,University of South,Hengyang 421001,China;Department of Radiology Medicine,School of Public Health,University of South China,Hengyang 421001,China)
出处
《国际放射医学核医学杂志》
2019年第3期257-262,共6页
International Journal of Radiation Medicine and Nuclear Medicine
基金
国家自然科学基金(81741143)
湖南省教育厅重点科研项目(17A186).
关键词
维生素E
Γ射线
辐射防护
黑腹果蝇
氧化性应激
Vitamin E
Gamma rays
Radiation protection
Drosophila melanogaster
Oxidative stress
