摘要
目的:探讨亚硝酸钠对人胚肾细胞HEK-293毒性机制。方法:将HEK-293人胚肾分别暴露于含0.0、3.0、6.0、12.0mmol/L Na2NO2培养液中24h,MTT法检测细胞活力,酶联免疫吸附法测定培养液中LDH、GGT、NAG、SOD、GSH、MDA含量。结果:低N组OD值、存活率高于对照组,中N组、高N组OD值、存活率低于对照组,差异有统计学意义(P<0.01);随着Na2NO2剂量的增加,HEK-293 OD值、细胞存活率先升高(P<0.01),然后明显降低(P<0.01);低N组LDH、GGT、NAG水平低于对照组,中N组、高N组LDH、GGT、NAG高于对照组,差异有统计学意义(P<0.01);随着Na2NO2剂量的增加,HEK-293 LDH、GGT、NAG先降低,后升高,剂量-效应关系明显(P<0.01);低N组SOD、GSH高于对照组,MDA低于对照组,中N组、高N组SOD、GSH低于对照组,MDA高于对照组,差异有统计学意义(P<0.01);随着Na2NO2剂量的增加,HEK-293 SOD、GSH先升高,后降低,MDA先降低,后升高,剂量-效应关系明显(P<0.01)。结论:低剂量亚硝酸钠对HEK-293细胞为毒物兴奋效应,中高剂量亚硝酸钠对HEK-293细胞表现为抑制作用,其机制与细胞膜通透性改变、线粒体损害以及氧化损伤有关。
Objective: To investigate the mechanism of sodium nitrite on the cytotoxicity of HEK-293 cells. Methods: HEK-293 human embryo kidneys were exposed to sodium nitrite containing 0.0, 3.0, 6.0, and 12.0 mmol/L Na2NO2, respectively for 24 h. Cell viability was measured by MTT assay, and enzyme-linked immunosorbent assay was used to determine the cell viability. LDH, GGT, NAG, SOD, GSH, MDA content in the liquid. Results: The OD value and survival rate of the low N group were higher than that of the control group. The OD values and survival rate of the N group and high N group were lower than the control group(P<0.01). With the increase of the Na2NO2 dose, HEK The OD value and cell survival rate first increased(P<0.01), then decreased significantly(P<0.01);the levels of LDH, GGT, and NAG in the low N group were lower than those in the control group;the LDH, GGT, and N in the N group and high N group. NAG was higher than the control group, and the difference was statistically significant(P<0.01). With the increase of Na2NO2 dose, HEK-293 LDH, GGT, and NAG first decreased, then increased, and the dose-effect relationship was significant(P<0.01). The SOD and GSH in the low N group were higher than those in the control group, and the MDA was lower than that in the control group. The SOD and GSH in the N group and the high N group were lower than those in the control group, and the MDA was higher than that in the control group(P<0.01). With the increase of Na2NO2 dose, HEK-293 SOD and GSH increased first and then decreased, MDA first decreased, then increased, and the dose-effect relationship was significant(P<0.01). Conclusion: Low-dose sodium nitrite is a toxic stimulatory effect on HEK-293 cells, and high-dose sodium nitrite inhibits HEK-293 cells, and its mechanism is related to changes in cell membrane permeability, mitochondrial damage, and oxidative damage.
作者
郑冲
刘泳廷
ZHENG Chong;LIU Yong-ting(Center for Disease Control and Prevention of Guizhou Province,Guiyang 550004,China)
出处
《微量元素与健康研究》
CAS
2019年第4期1-3,共3页
Studies of Trace Elements and Health
基金
贵州省疾病预防控制中心青年技术基金(2017-E1-3青)
