摘要
背景:皮肤光老化除受年龄因素影响外,与日光照射亦有直接关系,由日光照射引起的皮肤老化称为光老化。近年来,越来越多的研究发现多糖类物质对皮肤光老化具有一定的抑制作用,主要作用机制可能与其具有抗氧化、保湿、抗辐射等作用相关。目的:明确复合多糖抑制皮肤细胞光老化的作用是否优于单一多糖。方法:利用紫外灯照射诱导人皮肤成纤维细胞(HSF)、人永生化角质形成细胞(HaCaT)(均购自中国科学院上海生科院细胞资源中心)出现老化损伤。在此基础上,采用MTT法和生化试剂盒分别检测相同浓度下4种单一多糖(铁皮石斛多糖、褐藻多糖、灵芝多糖、竹荪多糖)和4种多糖混合物(即复合多糖)对2种光老化表皮细胞存活率及超氧化物歧化酶、丙二醛和羟脯氨酸水平的影响。结果与结论:①与模型组相比,铁皮石斛多糖、褐藻多糖、灵芝多糖、竹荪多糖在50mg/L的质量浓度下,对光老化人皮肤成纤维细胞模型及人永生化角质形成细胞模型的抑制效果均不显著,仅有个别生化指标改善程度显著(P<0.01或P<0.05);4种单一多糖对光老化细胞模型的保护作用相近;②与模型组比较,同样在多糖质量浓度为50mg/L时,复合多糖能够显著提高2种光老化细胞模型的存活率(P<0.01)、超氧化物歧化酶及羟脯氨酸水平(P<0.01或P<0.05),降低丙二醛水平(P<0.01或P<0.05);③结果表明,在对抗皮肤成角质细胞和成纤维细胞光老化实验中,4种单一多糖抗皮肤细胞光老化的效果不显著,而其混合物复合多糖(铁皮石斛多糖+褐藻多糖+灵芝多糖+竹荪多糖)抗皮肤细胞光老化的作用显著,提示复合多糖抑制皮肤细胞光老化的作用优于单一多糖。
BACKGROUND:In addition to aging,skin photoaging is directly related to sunlight exposure.Photoaging refers to skin aging caused by sunlight.In recent years,increasing evidence has shown that polysaccharides can certainly inhibit skin photoaging,as they have antioxidant,moisturizing,anti-radiation functions.OBJECTIVE:To explore whether complex polysaccharides are superior to single polysaccharides to inhibit photoaging of skin cells.METHODS:Irradiation by ultraviolet light was conducted to damage human skin fibroblasts(HSF)and human immortalized keratinocytes(HaCaT).Both kinds of cells were purchased from the Cell Bank of Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences.On the basis of this,MTT assay and biochemical kits were used to detect the survival rate,superoxide dismutase,malondialdehyde and hydroxyproline levels in HSF and HaCaT photoaging cell models after treated with four kinds of single polysaccharides(polysaccharide from Dendrobium officinale,polysaccharide from Phaeophyta,polysaccharide from Ganoderma Lucidum and polysaccharide from Dictyophora indusiata)and four kinds of polysaccharide mixtures(i.e.,complex polysaccharides)at the same concentration.RESULTS AND CONCLUSION:(1)Compared with the model group,the interventional results of single-component polysaccharides on HSF and HaCaT photoaging cells showed a significant improvement in some but not all biochemical indicators(P<0.01 or P<0.05).Four kinds of single polysaccharides had similar protective effects on photoaging cell models.(2)Compared with the model group,complex polysaccharides at 50 mg/L could significantly increase the cell survival rate and levels of superoxide dismutase and hydroxyproline,and decrease malondialdehyde level in HSF and HaCaT photoaging cell models(P<0.01 or P<0.05).To conclude,in the anti-ultraviolet-induced HSF and HaCaT photoaging experiments,the complex polysaccharides(polysaccharide from Dendrobium officinale+polysaccharide from Phaeophyta+polysaccharide from Ganoderma Lucidum+polysaccharide from Dictyophora indusiata)were better than the single polysaccharide of each component at the same intervention concentration.
作者
杨凯业
汪丽萍
王冰心
卢颖裕
冯姣
吴美音
施琬
侯少贞
Yang Kaiye;Wang Liping;Wang Bingxin;Lu Yingyu;Feng Jiao;Wu Meiyin;Shi Wan;Hou Shaozhen(Guangdong Infinitus (China) Co.,Ltd.,Guangzhou 510663,Guangdong Province,China;College of Chinese Materia Medica,Guangzhou University of Chinese Medicine,Guangzhou 510006,Guangdong Province,China;Guangzhou Hongyun Pharmaceutical Technology Co.,Ltd.,Guangzhou 510663,Guangdong Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2019年第29期4707-4713,共7页
Chinese Journal of Tissue Engineering Research
基金
广东省省级科技计划科技应用型科技研发专项资金项目(2015B020234008)
项目参与人:侯少贞~~
关键词
铁皮石斛多糖
褐藻多糖
灵芝多糖
竹荪多糖
成角质细胞
成纤维细胞
超氧化物歧化酶
羟脯氨酸
丙二醛
polysaccharide from Dendrobium officinale
polysaccharide from Phaeophyta
polysaccharide from Ganoderma Lucidum
polysaccharide from Dictyophora indusiata
keratinocyte
fibroblast
superoxide dismutase
hydroxyproline
malondialdehyde
