摘要
初步探讨SAB抑制乳腺癌增殖的剂量特征和机制.采用MTT法检测不同浓度SAB对人乳腺癌细胞MCF-7增殖的影响,进一步利用RT-PCR、Western blot等方法观察细胞周期蛋白、细胞凋亡相关因子、ER-α36、ER-α66等表达的变化.结果显示,低剂量(0.25mg/mL)的SAB促进MCF-7细胞的增殖,高剂量的SAB才能抑制MCF-7细胞的增殖,促进细胞凋亡,并且将细胞周期阻滞在S期.提示SAB通过影响不同ER亚型的表达介导不同的ER信号通路,从而起到对乳腺癌细胞的双相调节作用.
To investigate the dose characteristics and mechanism of SAB inhibition of breast cancer proliferation.In this paper,the effects of different concentrations of SAB on the proliferation of human breast cancer cell line MCF-7 were detected by MTT assay.The expressions of Cyclin,apoptosis-related factors,ER-α36 and ER-α66 were further observed by RT-PCR,Western blot.The dose characteristics and mechanism of SAB inhibition of breast cancer proliferation were preliminarily investigated.It was found that low dose(0.25 mg/mL)of SAB promoted the proliferation of MCF-7 cells,and high doses of SAB inhibited the proliferation of MCF-7 cells,promoted apoptosis,arrested the cell cycle in S phase.It is suggested that SAB may mediate different ER signaling pathways by affecting the expression of different ER subtypes,thereby regulating the biphasic function of breast cancer cells.
作者
邹伟
张若琳
孙佳
李洪艳
张叶军
张炳强
ZOU Wei;ZHANG Ruolin;SUN Jia;LI Hongyan;ZHANG Yejun;ZHANG Bingqiang(School of Life Science,Liaoning Normal University,Dalian 116081,China;Liaoning Key Laboratories of Biotechnology and Molecular Drug Research and Development,Dalian 116081,China;Key Laboratory of Cell Transplantation,Ministry of Health of the People’s Republic of China,Qingdao 266000,China)
出处
《辽宁师范大学学报（自然科学版）》
CAS
2019年第2期223-228,共6页
Journal of Liaoning Normal University:Natural Science Edition
基金
辽宁省科学技术计划项目(2015020568)
辽宁省教育厅科学技术研究项目(L201783647)
