摘要
目的探讨不同剂量帕瑞昔布对大鼠肺缺血再灌注(IR)后肺组织内血红素加氧酶-1(HO-1)表达水平及凋亡程度的影响。方法随机将40只雄性SD大鼠(280~300 g)分为5组:对照组(C组)、缺血再灌注组(IR组)、帕瑞昔布低剂量组(L组,2 mg/kg)、帕瑞昔布中剂量组(M组,10 mg/kg)及帕瑞昔布高剂量组(H组,20 mg/kg)。复制单侧肺IR模型,检测肺内丙二醛(MDA)和髓过氧化物酶(MPO)含量、肺组织湿重/干重,观察肺组织病理改变及凋亡情况,检测肺内HO-1表达。结果与C组比较,其他4组肺内MDA和MPO含量、肺组织湿重/干重、肺内凋亡细胞均增加(P <0.05),IR组增加最多,L组次之,M组和H组增加最少,且M组和H组差异无统计学意义(P>0.05)。肺IR后,IR组肺损伤最严重,不同剂量帕瑞昔布处理后,肺损伤均减轻,M组和H组较L组减轻更明显。C组内HO-1表达低,其余4组均升高(P <0.05),其中L组较IR组升高,M组和H组较L组进一步升高,M组和H组差异无统计学意义(P>0.05)。结论帕瑞昔布可减轻肺IR损伤,在一定程度呈剂量依赖性,其机制可能与增加肺组织中HO-1表达有关。
Objective To explore the effect of different doses of Parecoxib on expression of HO-1 and apoptosis in rat model of lung ischemia-reperfusion (IR). Methods Totally 40 male adult Sprague-Dawley rats were randomly divided into 5 groups: control group (group C), ischemia reperfusion group (group I/R), Parecoxib group (2 mg/kg, group L), parecoxib group (10 mg/kg, group M), and parecoxib group (20 mg/kg, group H). Pulmonary ischemia-reperfusion injury model was established by blocking the left hilum of lungs. The wet/dry weight ratio (W/D), MDA and MPO of lung tissues were measured. Apoptosis, histological morphology of lung tissue as well as the expression of HO-1 in lung were detected. Results MDA, MPO, W/D and apoptosis rate were increased significantly in group I/R when compared with those in normal group, which was attenuated by treatment of Parecoxib (P < 0.05). There was no significant difference in MDA, MPO, W/D and apoptosis rate between group M and group H (P > 0.05). Pulmonary injury was the most severe in group I/R, which was attenuated by different doses of Parecoxib in dose dependent manner. HO-1 was only slightly expressed in group C, while significant upregulation of HO -1 was detected in group I/R (P < 0.05) and was further elevated with treatment of Parecoxib. There was no statistical significant difference in HO -1 between group M and H (P > 0.05) Conclusions Parecoxib attenuates lung ischemia-reperfusion injury dose-dependently probably through increased expression of HO-1.
作者
吕强
刘宇
胡云霞
李美亭
Qiang Lü;Yu Liu;Yun-xia Hu;Mei-ting Li(Department of Anesthesiology, Sichuan Academy of Medical Sciences & Sichuan Provincial People’sHospital, Chengdu, Sichuan 610072, China)
出处
《中国现代医学杂志》
CAS
2019年第11期14-18,共5页
China Journal of Modern Medicine
基金
四川省卫生厅科研项目(No:110233)
