摘要
目的 分析赖氨酸特异性脱甲基酶2B(KDM2B)、B细胞淋巴瘤-2(Bcl-2)蛋白、Hrk蛋白在卵巢癌中的表达情况及其临床意义。方法 收集20例正常卵巢组织、30例良性卵巢肿瘤组织以及50例恶性卵巢肿瘤组织。采用免疫组织化学法检测3种组织KDM2B、Bcl-2蛋白、Hrk蛋白的表达情况。比较不同临床病理特征的恶性卵巢肿瘤患者间3种蛋白表达的情况,并分析恶性卵巢肿瘤组织中3种蛋白表达的相关性。结果 恶性卵巢肿瘤组织的KDM2B、Bcl-2蛋白阳性表达率高于良性卵巢肿瘤组织及正常卵巢组织(均P<0.05),恶性卵巢肿瘤组织、良性卵巢肿瘤组织、正常卵巢组织的Hrk阳性表达率依次升高(均P<0.05)。低分化、国际妇产科联合会(FIGO)分期Ⅲ~Ⅳ期、淋巴结转移病例的KDM2B及Bcl-2蛋白阳性表达率均分别高于中-高分化、FIGO分期Ⅰ~Ⅱ期、无淋巴结转移病例(均P<0.05),而Hrk蛋白阳性表达率均分别低于中-高分化、FIGO分期Ⅰ~Ⅱ期、无淋巴结转移病例(均P<0.05)。不同年龄段病例的3种蛋白阳性表达率比较,差异均无统计学意义(均P>0.05)。在卵巢恶性肿瘤组织中,KDM2B、Bcl-2蛋白与Hrk蛋白的阳性表达率均呈负相关(均P<0.05)。结论 卵巢癌中KDM2B与Bcl-2蛋白的表达上调,Hrk蛋白的表达下调,三者可能通过不同的方式参与卵巢癌的发生发展。
Objective To analyze the expression and clinical significance of lysine(K)-specific demethylase 2B(KDM2B),B-cell lymphoma-2(Bcl-2)protein,and Hrk protein in ovarian cancer tissues.Methods Twenty specimens of normal ovarian tissues,30 specimens of benign ovarian tumor tissues and 50 specimens of malignant ovarian tumor tissues were collected.The expression of KDM2B,Bcl-2 protein and Hrk protein from the three kinds of tissues were detected using immunohistochemistry assay.The expression of the three proteins were compared among patients with malignant ovarian tumors of different clinicopathological features,and the correlation among the three proteins expression in malignant ovarian tumor tissues was analyzed.Results The positive expression rates of KDM2B and Bcl-2 protein in malignant ovarian tumor tissues were higher than those in benign ovarian tumor tissues or normal ovarian tissues(all P<0.05),the positive expression rate of Hrk protein increased in the order of malignant ovarian tumor tissues,benign ovarian tumor tissues,and normal ovarian tissues(all P<0.05).Patients with poor differentiation,with International Federation of Gynecology and Obstetrics(FIGO)stageⅢ-Ⅳ,or with lymphatic metastasis had higher positive expression rates of KDM2B and Bcl-2 protein,along with lower positive expression rate of Hrk protein,than those with moderate-well differentiation,with FIGO stageⅠ-Ⅱ,or without lymphatic metastasis,respectively(all P<0.05).The positive expression rates of the three proteins showed no statistically significant differences among cases of various age groups(all P>0.05).In malignant ovarian tumor tissues,the positive expression rate of KDM2B or Bcl-2 protein negatively correlated with the positive rate of Hrk protein(all P<0.05).Conclusion Up-regulation of KDM2B and Bcl-2 protein,and down-regulation of Hrk protein are observed in ovarian cancer,the three proteins probably participate in the occurrence and development of ovarian cancer via various ways.
作者
易叶叶
况燕
蒙家华
杨慧兰
徐红
侯海瑞
YI Ye-ye;KUANG Yan;MENG Jia-hua;YANG Hui-lan;XU Hong;HOU Hai-rui(Department of Gynecology and Obstetrics,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)
出处
《广西医学》
CAS
2019年第10期1205-1209,共5页
Guangxi Medical Journal
基金
国家自然科学基金(81360389)
