摘要
为分析RNA识别区(RNA recognition motif,RRM)在RBM5蛋白调控肿瘤细胞周期中的作用,本研究利用低表达RBM5的A549细胞系构建了A549/Vector、A549/RBM5-WT、A549/RBM5-△RRM三种稳定细胞株,通过流式细胞术检测,发现仅有A549/RBM5-WT能显著阻滞细胞于G1期,说明RRM区是RBM5抑制细胞周期进程必不可少的区域.利用Western Blot检测细胞中cyclin A与Phospho-Rb(ser 795)的蛋白水平差异,证明了RBM5蛋白的RRM能够参与其介导的pRb去磷酸化以及抑制cyclin A表达,从而抑制细胞周期进程.
In order to analyze the role of RNA recognition region(RRM) in the regulation of tumor cell cycle by RBM5 protein, three stable cell lines A549/Vector, A549/RBM5-WT, A549/RBM5-△RRM were constructed by using A549 cell line with low expression of RBM5. The phase of cell cycle analysis by Flow cytometry showed that only A549/RBM5-WT was arrested in G1 phase, indicating that the RRM region was essential for RBM5 to inhibit cell cycle progression. Using Western Blot to detect the difference between cyclin A and Phospho-Rb(ser 795), it was proved that RRM of RBM5 protein could participate in pRb dephosphorylation mediated by RBM5 and inhibit cyclin A expression, thus inhibiting cell cycle progression.
作者
王迪
吴传芳
WANG Di;WU Chuan-Fang(Departement of Functional Genome, College of Life Sciences, Sichuan University, Chengdu 610065, China)
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2019年第3期531-536,共6页
Journal of Sichuan University(Natural Science Edition)
基金
国家自然科学基金(31300674)
