摘要
目的探讨常见毛囊细胞角蛋白在毛囊周期中的表达特征。方法取毛囊发育期、生长期启动、生长期、退化期和静止期的小鼠皮肤,石蜡切片后通过免疫荧光的方法,检测细胞角蛋白Krt5、Krt6、Krt10、Krt14、Krt15和Krt19的表达情况。结果 Krt5在静止期和生长期启动表达于所有毛囊上皮细胞,在其他时期表达不一致;Krt6表达于所有时期的外根鞘细胞和内根鞘细胞;Krt10表达于生长期和退化期的毛母质和内根鞘细胞,在其他时期表达不一致;Krt14在生长期和退化期表达于所有毛囊上皮细胞,在其他时期表达不一致;Krt15和Krt19表达于毛囊发育期、生长期启动和静止期的毛囊隆突区细胞,在生长期和退化期表达不一致。结论角蛋白作为毛囊结构或毛囊干细胞标记物仅适用于特定的毛囊周期。研究者在使用毛囊角蛋白作为标记物时,应首先明确其在毛囊周期中的表达情况。
Objective To evaluate the expression patterns of common hair follicle keratins in the hair follicle stages. Methods The mouse skins at different hair follicle stages including hair follicle morphogenesis, anagen onset, anagen, catagen, and telogen were harvested and embedded in paraffin. The sections were stained with anti-Krt5, anti-Krt6, anti-Krt10, anti-Krt14, anti-Krt15, and anti-Krt19 by immunostaining. Results Krt5 was expressed in the hair follicle epithelial of telogen and anagen stages. Krt6 was expressed in the outer root sheath and inner root sheath cells of hair follicles in all stages. Krt10 was expressed in the matrix and inner root sheath of anagen and catagen stages. Krt14 was expressed in the hair follicle epithelial of anagen and telogen stages. Krt15 and Krt19 were expressed in the bulge cells of morphogenesis, anagen onset, and telogen stages. Conclusion The keratins can only be used as hair structure or hair follicle stem cell markers at specific hair follicle stages. Researchers should determine the expression pattern of the hair follicle keratins in all hair follicle stages before using them as potential markers.
作者
燕荣帅
裴卓
马小艮
张一鸣
李玉红
Yan Rongshuai;Pei Zhuo;Ma Xiaogen;Zhang Yiming;Li Yuhong(Department of Plastic and Cosmetic Surgery,Xinqiao Hospital,Army Medical University,Chongqing 400037,China;Department of Cell Biology,Army Medical University,Chongqing 400038,China)
出处
《中华细胞与干细胞杂志（电子版）》
2019年第1期7-12,共6页
Chinese Journal of Cell and Stem Cell（Electronic Edition）
基金
国家自然科学基金面上项目(81472895)
重庆市自然科学基金项目(cstc2018jcyjAX0053)
关键词
角蛋白
毛囊
细胞周期
免疫荧光
Keratins
Hair follicle
Hair cycle
Immunofluorescence
