摘要
建立了超高效液相色谱法测定玉米低聚肽粉中AY含量的方法。样品超声萃取后过滤,WatersAcquityUPLCBEHC18色谱柱(50mm×2.1mm,1.7μm)分离,以0.1%三氟乙酸/乙腈为流动相,流速0.13mL/min,二极管阵列检测器,检测波长223nm。在2.5mg/L^40mg/L范围内呈线性相关,相关系数R为0.9999。样品中加标回收率为96.12%~107.07%,重现性RSD=1.04%。该法快速准确,简便灵敏,分离度高,能够满足玉米低聚肽中AY的检测要求。
A HPLC method for the determination of AY in corn oligopeptide powder was established. The sample was filtered by ultrasonic extraction and separated by the UPLC BEH C18 column (50mm×2.1mm, 1.7mm),Using 0.1%trifluoroacetic acrylonitrile as the flow phase, the flow rate was 0.13mL/min. Detector was diode array detector, detecting wavelength was 223nm. Linear correlation in range of 2.5mg/L~ 40mg/L, correlation coefficient R was 0.9999. The recovery rate was 96.12%~107.07%, and the reproducible RSD=1.04%. The method was fast, accurate, simple and sensitive, with high separation degree, and could meet the requirement of AY detection in corn oligopeptide.
作者
马雷
沈小梅
刘露
马金同
胡心行
刘国英
MA Lei;SHEN Xiaomei;LIU Lu;MA Jintong;HU Xinhang;LIU Guoying(Anhui GujingGongjiu Co. Ltd., Bozhou 236820, Anhui,China)
出处
《酿酒》
CAS
2019年第3期115-116,共2页
Liquor Making
关键词
超高效液相色谱法
AY
肽粉
ultra-high performance liquid chromatography
AY
Peptide powder
