摘要
为克服芽孢杆菌单株菌株在水产动物发酵饲料应用上的局限性和优化对虾颗粒饲料发酵条件,本试验通过对优选芽孢杆菌菌株进行配伍组合,使用福林酚法、3,5-二硝基水杨酸法和DNS法分别进行蛋白酶、淀粉酶以及纤维素酶活性的定量测定,将选出的酶活性较高的菌株,通过16S rDNA序列信息鉴定定种,并将各菌株进行配伍,以3种酶活性为指标优选最优组合。制备发酵对虾饲料,以酶活性为指标优化加水量、接种量、颗粒大小以及通气量。结果表明:在9种芽孢杆菌组合中,蛋白酶活性最高的组合为8-Z,酶活性为(28.58±0.34)U/mL、淀粉酶活性最高的组合为3-Z,酶活性为(5.58±1.10)U/mL、纤维素酶活最高的组合为3-Z,酶活性为(34.53±7.41)U/mL。菌株组合8-Z(包含解淀粉芽孢杆菌1-D、枯草芽孢杆菌3-D和枯草芽孢杆菌9-D)制备对虾发酵饲料的适宜参数为:加水量1 mL/g,接种量0.5×107～1.5×107 cfu/g,充气量20 mL/g,发酵时间24 h,在此条件下发酵后活菌数为6.48×108 cfu/g。
The present study aimed to overcome the restricion of the single strain of Bacillus in aquatic feed fermentation and to optimize the fermentation condition.Bacillus strains were evaluated by using the Folinol method,3,5-dinitrosalicylic acid method and DNS method to measure the activity of protease,amylase and cellulase,respectively.The selected strains with higher enzyme activity were identified by 16S rDNA sequence information.And their optimal combinations were determined by an integrated enzyme activity.Fermented prawns feed were subsequently prepared by the optimal Bacillus combination,and the water content,inoculation proportion,particle size and ventilation value were optimized using the enzyme activity as the index.The results showed that among the 9 Bacillus species combinations,the combination with the highest protease activity was 8-Z[(28.58±0.34)U/mL],the combination with the highest amylase activity was 3-Z[(5.58±1.10)U/mL],and the combination with the highest cellulase activity was 3-Z[(34.53±7.41 U/mL)].The suitable parameters for the preparation of shrimp fermentation feed by the combination of 8-Z(including Bacillus amyloidolysis 1-D,Bacillus subtilis 3-D and Bacillus subtilis 9-D)were as follows:shrimp feed fermented with 1 mL/g of water,0.5×10^7~1.5×10^7 cfu/g of inoculation,20 mL/g of aeration,fermentation time was 24 h,and the number of viable bacteria was 6.48×10^8 cfu/g.
作者
王竞儒
王洋
余政豪
乔秀亭
程镇燕
崔培
王庆奎
孙金辉
曹鹏
白东清
WANG Jingru;WANG Yang;YU Zhenghao;QIAO Xiuting;CHENG Zhenyan;CUI Pei;WANG Qingkui;SUN Jinhui;CAO Peng;BAI Dongqing(Aquatic apartment of Tianjin agricultural university,Tianjin 300384,China;Tianjin Baodi district Yuweng fishery science and technology development co.LTD,Tianjin 301800,China)
出处
《中国饲料》
北大核心
2019年第9期80-83,共4页
China Feed
基金
国家自然基金(青年科学基金项目)"乳酸片球菌素PA-1与乳酸协同损伤酸耐受嗜水气单胞菌内外膜的机理"(31702360)
国家级大学生创新创业训练计划项目(201710061005)
南美白对虾综合防控及时推广
天津市水产产业技术体系创新团队(ITTFRS2017003
ITTFRS2017004)
天津市高等学校"创新团队培养计划"现代水产生态健康养殖(TD13-5089)
天津市水产生态及养殖重点实验室平台项目
