摘要
目的探讨4-氨基丁酸转氨酶(ABAT)在精索静脉曲张大鼠睾丸中的表达和意义。方法无特定病原体(SPF)级雄性SD大鼠36只,将大鼠随机分为精索静脉曲张组和假手术组,各18只。采用左侧肾静脉半缩窄法结合完全结扎精索静脉各属支构建精索静脉曲张大鼠模型,于4周后采用苏木精-伊红(HE)染色、Masson及糖原染色评估两组肾脏、睾丸组织学改变,评估精索静脉曲张大鼠模型。模型成功构建后,采用免疫荧光分析ABAT蛋白在大鼠睾丸组织的定位,分别于4、6、8周后采用Western blot实验检测ABAT蛋白的表达变化及对生精影响。结果构建模型4周后,在精索静脉曲张组中肾脏组织均未发现病理学改变,且其左侧精索静脉直径大小均>1 mm[(1.52±0.23) mm比(0.26±0.03) mm],左侧睾丸重量[(1.61±0.08) g比(1.93±0.11) g]及生精小管的百分比(58%比18%)均低于对照组,这表明成功构建精索静脉曲张动物模型。免疫荧光分析可见ABAT蛋白主要表达在生精细胞胞质。与对照组比较,随着精索静脉曲张持续时间延长,Western blot实验发现ABAT蛋白表达在精索静脉曲张组中下调,这与生精受损程度呈正相关。结论在精索静脉曲张大鼠模型中,精索静脉曲张影响ABAT蛋白的表达,这可能与精索静脉曲张损害生精相关。
Objective To investigate the expression of 4-aminotransferase (ABAT) and its effect on spermatogenesis in experimental varicocele rat model. Methods A total of 36 specific pathogen free (SPF) male SD rats were randomly divided into varicocele group and sham operation group, with 18 rats in each group. The experimental rat varicocele was induced by partially occluding left renal vein and completely ligating the branches of the left spermatic vein (LSV). After 4 weeks, the using hematoxylin and eosin (HE) staining, Masson and glycogen staining were used to evaluate the two groups of kidney and testis histology change, evaluation of varicocele rat model. After the model was constructed, the localization of ABAT protein in rat testis was further analyzed by immunofluorescence, and the expression of ABAT protein in varicocele was detected by Western blotting, and the possible role was analyzed. Results 4, 6, 8 weeks after the operation, the varicocele group in the kidney tissue pathological changes were not found, and the left spermatic vein diameter was greater than 1 mm [(1.52±0.23) mm vs.(0.26±0.03) mm], the weight of the left testis [(1.61±0.08) g vs.(1.93±0.11) g] and the percentage of seminiferous tubules (58% vs. 18%) were lower than the control group, which indicates that the successful construction of varicocele animal model. Immunofluorescence analysis showed that ABAT protein was mainly expressed in the cytoplasm of spermatogenic cells. Compared with the control group, with the prolonged duration of varicocele, Western blotting showed that the expression of ABAT protein was down regulated in the varicocele group, which is positive with the degree of spermatogenesis damage. Conclusion In experimental rat varicocele model, varicocele affects the expression of ABAT protein, which may be related to varicocele damage spermatogenesis.
作者
杨诚
郭文彬
张万松
郭晓彬
王鹏
肖卓裕
杨建昆
夏慧
Yang Cheng;Guo Wenbin;Zhang Wansong;Guo Xiaobin;Wang Peng;Xiao Zhuoyu;Yang Jiankun;Xia Hui(Department of Urology, the Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, China)
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2019年第4期702-705,共4页
Chinese Journal of Experimental Surgery
基金
2017年广东省科技计划项目(2017ZC0090).
