摘要
目的探讨N,N'-二乙酰胱氨酸(DiNAC)对四氯化碳(CCl4)诱导大鼠肝纤维化的保护作用。方法采用随机数字表法将90只大鼠分为正常组、模型组、DiNAC低剂量组、DiNAC中剂量组、DiNAC高剂量组、二硫氨基甲酸肽吡咯烷(PDTC)组(作为阳性对照)6组,每组15只。后5组大鼠采用50%CCl4按1ml/kg皮下注射2次/周,连续10周制备大鼠肝纤维化模型。建模开始第2周起DiNAC低、中、高剂量组大鼠分别腹腔注射DiNAC12.5、25、50 mg/(kg·d),PDTC组大鼠腹腔注射PDTC100mg/kg,连续8周。通过检测血液生化指标及胶原、抗氧化指标,观察肝脏形态及组织结构,对DiNAC治疗大鼠肝纤维化的疗效进行评价。结果与模型组比较,DiNAC治疗组血清学指标AST、ALT和ALP明显改善,血清胶原指标下降,肝组织丙二醛含量下降,超氧化物歧化酶活性增加;DiNAC各组HE、网状纤维染色结果显示肝细胞脂肪变性、炎症、坏死及肝纤维化均有所好转。结论 DiNAC具有抗氧化作用,对大鼠肝纤维化具有较好的治疗作用。
Objective To investigate the effect of N,N'-diacetyl-L-cystine(DiNAC)on hepatic fibrosis induced by carbon tetrachloride.Methods Ninety male SD rats were randomly assigned into the control group,model group,low-dose group,medium-dose group,high-dose group and PDTC(pyrrolidine dithiocarbamate)group with 15 rats in each group.Hepatic fibrosis was induced by subcutaneous injection of 1ml/kg carbon tetrachloride(CCl4)for 10 wks in model group and 4 treatment groups.DiNAC(12.5,25,50mg/kg)or PDTC(100mg/kg)were intraperitoneally injected q.d from w2 in rats of low-,medium-,high-dose groups and PDTC group,respectively.After 8-w treatment,the rats were sacrificed,the hepatic tissue was examined histopathologically with HE and reticular fiber staining;blood biochemistry,collagen,antioxidant index were determined.Results Compared with the model group,DiNAC significantly improved the serological indexes and serum collagen indexes,decreased MDA level and increased SOD level;and also attenuated the severity of liver fibrosis histopathologically.Conclusion DiNAC has a protective effect on liver fibrosis induced by CCl4 in rats,which may be associated with its antioxidative effect.
作者
叶坚虹
王福根
缪樑斌
席建军
孙晶晶
YE Jianhong;WANG Fugen;MIAO Liangbin(Department of Liver Disease,Hangzhou Xixi Hospital Affiliated to Zhejiang Chinese Medical University,Hangzhou 310023,China)
出处
《浙江医学》
CAS
2019年第8期776-779,共4页
Zhejiang Medical Journal
基金
杭州市科技发展计划项目(20140633B28)
杭州市医药卫生科技计划项目(2014A44)
