摘要
目的探讨亚砷酸钠(NaAsO_2)对神经细胞周期的影响及MPST过表达在其中的作用。方法以空白组(正常SH-SY5Y细胞,BC)、空载对照组(NC)、过表达组(过表达MPST,OP)细胞分别经NaAsO_2处理后,采用CCK-8法、结晶紫染色法、流式细胞术和Western blot法,分别检测细胞活力、细胞贴壁力、细胞周期及p53、CDC25A、CyclinA、CDK2等蛋白表达水平。结果经NaAsO_2处理48 h后,NC组细胞存活率、细胞贴壁率明显降低,而过表达MPST细胞上述变化明显得到改善(P<0.01)。同时,NaAsO_2明显上调BC、NC组细胞S期比例和p53蛋白表达,而下调CDC25A、CyclinA及CDK2蛋白(P<0.01);然而,过表达MPST则明显拮抗上述效应(P<0.05,P<0.01)。结论 NaAsO_2通过诱导SH-SY5Y细胞S期阻滞,抑制细胞生长,而过表达MPST可拮抗NaAsO_2诱导的毒性效应。
Aim To investigate the effects of sodium arsenite (NaAsO2) on the cell cycle and growth, and the intervention of MPST over-expression in the neural cells. Methods NaAsO2 was used to treat SH-SY5Y neuroblastoma cells for 48 h from the blank control (BC),empty vector control (transfected with empty vector,NC) and over-expression group (lentiviral transfection with MPST,OP).The methods of CCK-8,crystal violet staining,flow cytometry and Western blot were used to examine cell viability,adherent rate,cell cycle and protein expression of p53,CDC25A,CyclinA and CDK2. Results The cell viability and adherent rate significantly decreased after treatment with NaAsO2 for 48 h,which was reversed in OP group ( P <0.01).Meanwhile,NaAsO2 also significantly increased the proportion of S phase cells and p53 protein expression,and down-regulated the protein levels of CDC25A,Cyclin A and CDK2 in BC and NC groups ( P <0.01),whereas the above changes of protein levels were significantly antagonized in OP group compared with NC group( P<0.05,P <0.01). Conclusions NaAsO 2 inhibits the cell growth by inducing S-phase arrest and over-expression of MPST could reverse the noxious effects caused by NaAsO2 in SH-SY5Y cells.
作者
柳香香
孙达权
许键炜
范海琼
徐国强
潘际刚
LIU Xiang-xiang;SUN Da-quan;XU Jian-wei;FAN Hai-qiong;XU Guo-qiang;PAN Ji-gang(School of Basic Medical Science,Guizhou Medical University,Guiyang550025,China;Experimental Centerof Stem Cell and Tissue Engineering,Guizhou Medical University,Guiyang550004,China;QiandongnanVocational and Technical College for Nationalities,Kaili Guizhou556000,China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2019年第5期634-639,共6页
Chinese Pharmacological Bulletin
基金
中国医学科学院成体干细胞转化研究重点实验室开放性课题(No 2018YB03)
贵州省科技厅贵阳医学院联合基金(黔科合LG字[2012]018号)
贵阳市科技计划项目(筑科合同[20141001]38号)
