摘要
该研究采用培养法与荧光显微计数法对方斑东风螺(Babylonia areolata)养殖水体和底沙的细菌数量进行了调查,并监测主要环境因子,包括水温、盐度、pH、溶解氧、水体氨氮(NH_4^+-N)、亚硝酸盐氮(NO_2^-N)、底质总氮(TN)、总磷(TP),旨在掌握方斑东风螺养殖环境中细菌数量变化情况,并探讨细菌数量与环境因子的关系。结果显示,东风螺养成期水体总细菌(WTB)、异养菌(WHB)、弧菌(WV)数量分别为:4.15×10~4—4.36×10~6cells·mL^(-1)、4.13×10~3—9.67×10~5 CFU·m L^(-1)、1.83×10~2—1.42×10~4 CFU·m L^(-1);底沙中异养细菌(SHB)和弧菌(SV)数量分别为:1.26×10~6—3.69×10~7 CFU·g^(-1)和6.25×10~4—2.07×10~6 CFU·g^(-1);水体总细菌显著高于水体及底沙中异养菌及弧菌数。相关性分析结果表明,水体总细菌数量与溶解氧(P<0.01)和亚硝酸盐氮(P<0.05)呈显著负相关,与氨氮呈正相关(P<0.05);水体异养菌与盐度呈显著正相关(P<0.05),与溶解氧极显著负相关(P<0.01);水体弧菌数量与温度呈负相关(P<0.05);底沙弧菌数量与温度呈极显著负相关(P<0.01),与盐度呈显著正相关(P<0.05),底沙异养菌(SHB)与各环境因子无明显相关性。主成分分析结果表明,温度与溶解氧在该养殖系统中占重要地位,不同时期环境的主导因子存在差异。
Heterotrophic bacteria and vibrio quantity in aquaculture system of Babylonia areolata were measured by cultivation method,and total bacteria in water(WTB)were measured by fluorescence microscopy.In addition,we investigated the main environmental factors including temperature,salinity,pH,dissolved oxygen and major nutritive salt of water and sediment,for the aim to determine the relationship between bacterial quantity and environmental factors.The results showed that the quantity of WTB was 4.15×10^4 to 4.36×10^6 cells·mL^-1,that of heterotrophic bacteria of water(WHB)was 4.13×10^3 to 9.67×10^5 CFU·mL^-1 and of sediment(SHB)was 1.26×10^6 to 3.69×10^7 CFU·g^-1,that of Vibrio of water(WV)was 1.83×10^2 to 1.42×10^4 CFU·mL^-1 and Vibrio of sediment(SV)was 6.25×10^4 to 2.07×10^6 CFU·g^-1.Correlation analysis results showed that the quantity of WTB was negative correlation with both dissolved oxygen(P<0.01)and nitrite(P<0.05),and positively correlated with ammonia nitrogen(P<0.05).WHB was positive correlation with salinity(P<0.05)and negative correlation with dissolved oxygen(P<0.01).WV was negative correlation with temperature(P<0.05),and others were not obvious.Quantity of SV was negative correlation with temperature(P<0.01)and positive correlation with salinity(P<0.05).The correlations of SHB with environment factors were inconspicuous.Principal component analysis(PCA)showed that the temperature and dissolved oxygen played important roles in the farming system and the dominant factors of environment differed in different periods.
作者
凌慧
赵旺
王瑞旋
黄晟
刘晓靖
吴开畅
王江勇
LING Hui;ZHAO Wang;WANG Ruixuan;HUANG Sheng;LIU Xiaojing;WU Kaichang;WANG Jiangyong(Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization,Ministry of Agriculture,South ChinaSea Fisheries Research Institute,Guangzhou 510300,China;College of Fisheries and Life,Shanghai Ocean University,Shanghai 201306,China)
出处
《生态科学》
CSCD
2019年第2期130-137,共8页
Ecological Science
基金
现代农业产业技术体系建设专项资金-贝类产业技术体系科学家岗位专项经费项目(CARS-48)
中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目(2015YD04)
广东省渔港建设和渔业产业发展专项(海洋渔业科技推广方向-科技攻关与研发项目(A201601B11)
海南省应用技术研发与示范推广专项(ZDXM2015058)
海南省自然科学基金项目(314180
20153170)
三亚市农业科技创新项目(2015KJ05)
关键词
方斑东风螺
细菌
环境因子
相关性
主成分分析
Babylonia areolate
bacteria
environmental factors
correlation
principal component analysis
