摘要
为了优化建立火棘多糖铁(Ⅲ)复合物(PPC)中铁含量检测的方法。考察了邻菲啰啉溶液浓度(w/w,%)及用量(mL)、抗坏血酸溶液浓度(w/w,%)及用量(mL)、反应时间(h)及温度(℃)等因素对PPC中铁含量测定的影响。在单因素实验的基础上,运用响应面软件进一步优化PPC的检测条件。结果表明,PPC中铁含量的最佳检测条件为:向1.0 mL适宜浓度的PPC溶液中,依次加入1.5 mL 10%的抗坏血酸溶液及3.0 mL 0.1%的邻菲啰啉溶液,于45℃水浴2.0 h后,冰水快速冷却,在室温下于510 nm波长处测其吸光度。该方法具有良好的重复性及重现性,回收率达99.93%。此方法也适合推广于其他植物多糖铁复合物中铁含量的测定。
In order to get the optimum conditions for determination of iron in Pyracantha polysaccharide-Fe (III) complex (PPC).The effects of O -phenanthroline concentration (w/w,%) and its amount (mL),ascorbic acid concentration (w/w,%) and its amount (mL),reaction time (h) as well as its temperature (℃) on the determination of iron in PPC were investigated.On the basis of single factor experiment,response surface software was used to further optimize the detection conditions of PPC.The results showed that the optimum conditions for the determination of iron content in PPC were as follows:1.5 mL 10% ascorbic acid and 3.0 mL 0.1% O -phenanthroline solution were added successively to the PPC solution of 1.0 mL of suitable concentration,and the absorbance was measured at 510 nm at room temperature after a water bath at 45 ℃ for 2.0 h.The developed method had good reproducibility and reproducibility with a recovery rate of 99.93%,which can be applied to detect iron content in other plant polysaccharide- iron complexes.
作者
蒋莉
付启明
王磊
蔡易睿
钟方旭
鄢又玉
JIANG Li;FU Qi-ming;WANG Lei;CAI Yi-rui;ZHONG Fang-xu;YAN You-yu(School of Biological and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan 430023,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2019年第4期648-654,共7页
Natural Product Research and Development
基金
国家自然科学基金子课题(21602166)
关键词
火棘
多糖铁复合物
含量检测
响应面优化
Pyracantha fortuneana
polysaccharide-Fe complex
content determination
response surface optimization
