摘要
为了研究拐枣总黄酮(TF)最佳提取工艺及其体外抗黄嘌呤氧化酶(xanthine oxidase,XO)活性。在单因素试验基础上,实验选择料液比、提取时间和乙醇浓度为自变量,应用Box-Benhnken中心组合法进行3因素3水平试验设计,以拐枣总黄酮得率为响应值,进行响应面分析,并采用D101大孔树脂对总黄酮进行进一步富集纯化,在此基础上研究拐枣总黄酮体外抗XO活性。结果表明,拐枣总黄酮的最佳提取条件为料液比15.0 mL/g、提取时间1.85 h、乙醇浓度53.0%,在此条件下,拐枣总黄酮的平均提取率可达到2.56%。体外活性结果表明,我们首次发现拐枣总黄酮具有显著的抗XO活性,其IC_(50 )为18.63±1.67μg/mL。本研究可为从拐枣中寻找和发现天然抗XO活性分子提供参考,同时可为拐枣的开发利用提供思路。
In order to optimize extraction of total flavonoids from Hovenia acerba and investigate xanthine oxidase inhibitory activity.Based on the results of single-factor tests,the ratio of liquid to solid,extraction time and ethanol concentration were optimized by response surface analysis.The results showed that the process of optimum extraction were the ratio of liquid to solid of 15:1 (mL:g),ethanol concentration of 53%,extraction time of 1.85 h.Under these conditions,the extraction yield of total flavonoids was 2.56%,which was close to the predicted value.The total flavonoid (TF) was further purified by D101 macroporous resin and evaluated for its xanthine oxidase (XO) inhibitory activities in vitro ,and the results showed that TF possessed significant enzyme inhibitory action with IC 50 value of 18.63 ± 1.67 μg/mL.These results indicated that the optimized extraction technology was reliable and provided a reference for further development and utilization of the Hovenia acerba.
作者
许洪波
李铂
唐志书
李圆
冀春
刘乃尧
吴群军
王薇
XU Hong-bo;LI Bo;TANG Zhi-shu;LI Yuan;JI Chun;LIU Nai-yao;WU Qun-jun;WANG Wei(Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization;College of Pharmacy,Shaanxi University of Chinese Medicine,Xianyang 712046 ,China;Xunyang Taijiyuan Biotechnology Co.,Ltd.,Ankang 725741 ,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2019年第4期595-600,716,共7页
Natural Product Research and Development
基金
国家自然科学基金(81603284)
陕西省教育厅自然科学研究项目(18JK0214)
陕西省重点科技创新团队(2012KTC-20)
关键词
拐枣
黄酮
响应面法
黄嘌呤氧化酶
Hovenia acerba
flavonoid
response surface methodology
xanthine oxidase
