摘要
普鲁兰多糖在食品、医药、化工等行业应用广泛.糖脂中间体lipid-G是普鲁兰多糖合成的基本单元,它由脂类物质和尿苷二磷酸葡萄糖(UDPG)结合而成.载脂蛋白负责调控脂类物质的合成,结果表明载脂蛋白基因apo和gltP能够调节普鲁兰多糖合成,整合过表达apo或gltP能使多糖产量分别增加7.78%和15.23%,而敲除apo或gltP则使多糖产量分别降低21.35%和35.07%.强化普鲁兰多糖合成的传统思路多从增加UDPG供给入手,而本研究发现,通过调控脂类物质来增强糖脂中间体lipid-G的合成也能够促进普鲁兰多糖的生产,这为通过代谢工程手段强化普鲁兰多糖合成提供了新的思路.
Pullulan has been widely used in food, pharmaceutical, and chemical industry. The glycolipid intermediate lipid-G is the basic unit for pullulan synthesis, and it was formed through combining lipid and UDPG. Appoliporoptein can regulate the synthesis of lipid. In the present study, the effect of two kinds of apolipoprotein genes(apo and gltP)on pullulan synthesis was examined. It was indicated that apo and gltP can regulate pullulan synthesis, but the molecular weight and the structure of the pullulan produced was not regulated by apo and gltP. Overexpression of apo or gltP could result in 7.78% and 15.23% increase in pullulan production, respectively, while the pullulan production decreased by 21.35% and 35.07% respectively after deletion of apo or gltP. In order to strengthen the formation of pullulan, the traditional method is to increase the amount of UDPG. In this research, we found out that by regulating the lipid synthesis, the glycolipid intermediate can be increased, thus leading to an increase in pullulan production. The present work provides a new way for increasing pullulan production through metabolic engineering.
作者
郭建
黄思瑶
郑鹏
陈叶福
郭学武
肖冬光
GUO Jian;HUANG Siyao;ZHENG Peng;CHEN Yefu;GUO Xuewu;XIAO Dongguang(Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,College of Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China)
出处
《天津科技大学学报》
CAS
2019年第2期12-18,共7页
Journal of Tianjin University of Science & Technology
基金
长江学者和创新团队发展计划资助项目(IRT1166)
"十二五"国家科技计划农村领域资助项目(2012AA101805)
