摘要
探明枸杞对盐胁迫的生理响应,为今后更好地利用枸杞改良盐碱地和生产高品质的枸杞子提供理论基础。以枸杞的优良品种(海杞)幼苗为试验材料,采用生理生化方法,分析不同盐浓度(0,50,100,150 mmol/L)、不同时间(100 mmol/L Na Cl处理下的第0,1,5,10 d)的盐胁迫下SOD,CAT,POD,APX等抗氧化酶的活性,以及过氧化产物(MDA)、可溶性糖类、脯氨酸及可溶性蛋白的含量。结果表明,随盐浓度升高,枸杞叶片和根中的细胞膜氧化伤害显著增多,且叶片受到的伤害比根严重。不同盐浓度的处理下,除了SOD、叶片和根受CAT,APX和POD重要的保护作用,但在两者之间的作用不同;且可溶性糖、可溶性蛋白质和脯氨酸迅速积累。在较长时间的盐胁迫条件下(5,10 d),后期抗氧化酶(SOD,CAT,POD)及渗透调节物质(如可溶性蛋白质和脯氨酸)的累积可以保证枸杞耐受长时间的盐胁迫。不同盐浓度和不同时间盐处理下,渗透调节物质(特别是可溶性蛋白质和脯氨酸)和抗氧化酶协同作用清除活性氧自由基,调节了枸杞对盐胁迫的响应,降低了氧化伤害,提高了枸杞的耐盐性,说明枸杞对盐胁迫具有一定的耐受性,适合在盐碱地种植。
To elucidate the physiological mechanisms of Lycium barbarum L. in response to salt stress,we investigated the antioxidant responses to salt stress in seedlings of Lycium barbarum L.'Haiqi' under different salt concentrations(0,50,100,150 mmol/L)and different periods(100 mmol/L NaCl lasted 1,5,10 d). Respectively,by examining the antioxidative enzyme activities,contents of soluble sugar,MDA,proline,and soluble proteins. Leaves and roots in Lycium barbarum L. seedlings were protected by CAT,APX and POD,rather than SOD,under different salt concentrations,but the protective effects of each of them differed between leaves and roots. Moreover,soluble sugar,soluble protein and proline rapidly accumulated. In response to the time of salt stress,the increased activities of antioxidant enzymes(SOD,CAT,POD)and accumulation of the osmotic regulators(such as soluble protein and proline)in the later period(5,10 d)can attribute to the salt resistance of Lycium barbarum L. seedlings. Overall,in response to different concentrations of salt stress and stress period,osmotic regulators(especially soluble protein and proline)and antioxidant system cooperated to scavenge reactive oxygen species(ROS),regulated the response of Lycium barbarum L. to salt stress,reduced oxidative damage,and improved the salt tolerance of Lycium barbarum L.,indicating that Lycium barbarum L. has a certain tolerance to salt stress. It is suitable for planting in saline alkali soil.
作者
白丽丽
李玉龙
巩健
李豪
谢志霞
王文颖
徐进
BAI Lili;LI Yulong;GONG Jian;LI Hao;XIE Zhixia;WANG Wenying;XU Jin(College of Life Science and Geography,Qinghai Normal University,Xi'ning,Qinghai 810008,China;Pharmaceutical and Biological Engineering Department,Zibo Vocational Institute,Zibo,Shandong 255314,China;Center for Agricultural Resources Research,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Shijiazhuang,Hebei 050022,China;College of Forestry,Shanxi Agricultural University,Jinzhong,Shanxi 030801,China;Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences,Kunming,Yunnan 666303,China)
出处
《农产品加工》
2019年第8期50-56,共7页
Farm Products Processing
基金
青海省创新平台建设专项项目(2017-ZJ-Y20)
关键词
枸杞
盐胁迫
氧化伤害
抗氧化酶
渗透调节
Lycium barbarum L
salt stress
oxidative damage
antioxidant enzymes
osmoregulation
