摘要
目的通过比较第三代杂交捕获技术定量分型(daltonbio hybrid capture 3, DH3)与第二代杂交捕获技术(the second generation of hybrid capture, HC2) 2种方法检测人乳头状瘤病毒(human papiloma virus,HPV)的结果,探讨DH3对意义不明的非典型鳞状细胞(atypical squamous cells of undetermined significance,ASCUS)分流的应用价值。方法采用DH3、HC2分别检测198例昆明医科大学第二附属医院行薄层液基细胞学检测技术(Thin-Cytologic Test, TCT)检查提示ASCUS患者宫颈脱落细胞样本中的高危HPV,并行阴道镜下宫颈活检,分析DH3与HC2检测结果的符合率及对ASCUS分流的情况。结果 (1) DH3与HC2两种方法检测高危型HPV总符合率为96.43%,阳性符合率为96.67%,阴性符合率为96.30%;(2)病理检查结果:CIN1 23例(11.62%); CIN2 30例(15.15%); CIN3 3例(1.51%);宫颈癌1例(0.51%);CIN2及以上病变检出率为17.17%;(3) CIN2及以上病变共34例,全部HPV感染阳性,其中29例DH3中HPV16/18阳性。结论 DH3与HC2检测符合率高,DH3中2种高危亚型对ASCUS患者的分流具有更重要的指导意义,可作为临床上ASCUS分流的有效手段。
Objective To explore the value of daltonbio hybrid capture 3(DH3) in ASCUS shunt by comparing the second generation of hybrid capture(HC2)and DH3 detection results.Methods The high-risk HPV subtypes were detected by DH3 and HC2 respectively from ASCUS population at the Second Affiliated Hospital of Kunming Medical University hospital with 198 cases.Cervical biopsy under colposcope,The coincidence rate of DH3 and HC2 test results and the triage of ASCUS were analyzed. Results(1)The total coincidence rate of high-risk HPV detected by DH3 and HC2 was 96.43%, 96.67% positive and 96.30% negative.(2)The pathological results were as follows: CIN1, CIN2, CIN3,cervical cancer was respectively 23 cases, 30 cases, 3 cases, 1cases,.The detection rate of CIN2+ was 17.17%.(3)CIN2+ were 34 cases , all of which were positive for HPV infection, of which 29 cases were positive for hpv16/18. Conclusions The coincidence rate between DH3 and HC2 is high.The two high-risk subtypes of DH3 have more important guiding significance for the triage of ASCUS patients, and can be used as an effective method for clinical triage of ASCUS.
作者
孙春意
许长俊
黄娅娟
卿清
李岱株
刘洋
SUN Chun-yi;XU Chang-jun;HUANG Ya-juan;QING Qing;LI Dai-zhu;LIU Yang(Dept. of Gynecology,The Second Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650101,China;Dept. of Reproductive,The Second Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650101,China)
出处
《昆明医科大学学报》
CAS
2019年第2期49-53,共5页
Journal of Kunming Medical University
基金
国家自然科学基金资助项目(81860515)
云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目[2018FE001(-055)]
云南省卫计委医学后备人才培养项目(H-201629)
云南省卫生科技计划项目(2016NS287
2017N2 87
2016NS286
2014NS092
2014NS091)
