摘要
目的评估二代测序(NGS)技术在检测感染性心内膜炎(IE)患者瓣膜中潜在病原菌的应用价值,为IE的诊断和术后治疗提供帮助。方法根据改良Duke标准,对IE患者和排除IE患者的瓣膜组织进行传统培养,同时应用NGS技术直接检测瓣膜组织上的病原菌,并将其检测结果与血培养、病理涂片染色结果进行比对。结果 NGS的灵敏度、特异度、阳性预测值、阴性预测值分别为95.0%、85.7%、95.0%和85.7%,血培养分别为30.0%、100.0%、100.0%、30.0%,瓣膜培养为10.0%、100.0%、100.0%、28.0%。结论与传统培养方法相比,NGS检测IE瓣膜赘生物的灵敏度更高,时间短。NGS对IE的诊断,尤其是培养结果为阴性疑似IE的诊断和治疗有较大的应用价值。
Objective To evaluate the value of next-generation sequencing(NGS)technique in detecting potential pathogens in valves of patients with infective endocarditis(IE),and provide help for the diagnosis and postoperative treatment of IE.Methods According to modified Duke criteria,valve tissues of IE patients and patients who were excluded IE were performed culture conventionally,pathogens in valve tissues were detected directly by NGS technique,results were compared with blood culture and pathological smear staining.Results The sensitivity,specificity,positive predictive value,and negative predictive value of NGS were 95.0%,85.7%,95.0%,and 85.7%respectively,blood culture were 30.0%,100.0%,100.0%,and 30.0%respectively,valve culture were 10.0%,100.0%,100.0%,and 28.0%respectively.Conclusion Compared with conventional culture method,NGS technique has higher sensitivity and shorter time in detecting IE valve vegetation.NGS is of great value in the diagnosis and treatment of IE,especially in suspected IE with negative culture results.
作者
程军
胡欢
张思明
耿洁
李峰
王飞燕
周洲
陈曦
CHENG Jun;HU Huan;ZHANG Si-ming;GENG Jie;LI Feng;WANG Fei-yan;ZHOU Zhou;CHEN Xi(Laboratory Diagnostic Center,Peking Union Medical College & Fuwai Hospital Chinese Academy of Medical Sciences,Beijing 100037,China)
出处
《中国感染控制杂志》
CAS
CSCD
北大核心
2019年第4期277-282,共6页
Chinese Journal of Infection Control
基金
重大协同创新项目(2016-12M-1-016)
关键词
二代测序
感染性心内膜炎
心脏瓣膜
诊断
next-generation sequencing
infective endocarditis
heart valve
diagnosis
