摘要
Short coleoptiles associated with GA-insensitive Rht-1 alleles in wheat reduces yield due to poor seedling establishment under dry,or stubble-retained conditions.Hence there is a need for alternative dwarfing genes for wheat improvement programs.GA-sensitive dwarfing gene Rht14 confers semidwarf stature in wheat while retaining longer coleoptiles and early seedling vigor.Two RIL populations were used to identify the map position of Rht14 and to estimate its effect on plant height,coleoptile length,seedling shoot length,spike length and internode length.Rht14 on chromosome 6 A was mapped in the genomic region 383–422 Mbp flanked by GA2oxA9 and wmc753 in a Bijaga Yellow/Castelporziano RIL population.Recombination events between Rht14 and GA2oxA9 in the RIL population indicated that Rht14 might not be allelic to GA2oxA9.The conserved DNA sequence of GA2oxA9 and its flanking region in Castelporziano also suggested that the point of mutation responsible for the Rht14 allele must be a few Mbp away from GA2oxA9.The dwarfing effects of Rht14 on plant height,internode length and seedling vigor were compared with those of Rht-B1 b in an HI 8498/Castelporziano RIL population.Both genes significantly reduced plant height and internode length.Rht-B1 b conferred a significant reduction in coleoptile length and seedling shoot length,whereas Rht14 reduced plant height,but not coleoptile and seedling shoot length.Therefore,Rht14 can be a used as an alternative to Rht-B1 b for development of cultivars suitable for deeper sowing in dry environments and in conditions of conservation agriculture where crop residues are retained.
基金
Financial support by the Science and Engineering Research Board,New Delhi under a Start-Up Research grant for Young Scientists(SB/FT/LS-243/2012)to Ravindra Patil
