摘要
目的观察雌激素和黑升麻对去势大鼠下颌下腺神经型一氧化氮合酶(neuronal nitric oxide synthase,nNOS)及瞬时受体电位香草酸亚型1(transient receptor potential vanilloid 1,TRPV1)表达的影响,探讨雌激素和黑升麻的作用机制,为临床用药提供参考。方法将成年SD雌性大鼠40只分为对照组(假手术)、去卵巢组(去除卵巢)、雌激素治疗组(去除卵巢+雌激素治疗)和黑升麻治疗组(去除卵巢+黑升麻治疗),每组10只。大鼠术后恢复2周,对照组和去卵巢组用蒸馏水,雌激素和黑升麻治疗组分别用β-雌二醇和黑升麻治疗,4周后免疫组化法比较各组大鼠下颌下腺nNOS、TRPV1、M1和M3型毒蕈碱型乙酰胆碱受体(muscarinic acetylcholine receptor,member 1 and 3,简称M1和M3受体)的表达。利用乙酰胆碱酯酶(acetylcholinesterase,AChE)染色比较各组大鼠下颌下腺AChE的表达。结果去卵巢组nNOS、TRPV1、M1和M3受体表达(0.16±0.01、0.21±0.01、0.15±0.02、0.09±0.02)较对照组(0.23±0.02、0.28±0.01、0.25±0.03、0.19±0.03)均显著降低(P<0.05);雌激素治疗组(0.23±0.01、0.28±0.02、0.23±0.03、0.19±0.01)与对照组差异无统计学意义(P>0.05);但黑升麻治疗组与去卵巢组相比,nNOS、TRPV1和M3受体表达未见显著改变,仅M1受体表达(0.22±0.03)显著增加(P<0.05)。去卵巢组和雌激素治疗组AChE表达(0.14±0.01和0.15±0.01)较对照组(0.10±0.01)显著增加(P<0.05);黑升麻治疗组AChE表达(0.09±0.01)与对照组差异无统计学意义(P>0.05)。结论雌激素可显著增加大鼠下颌下腺nNOS和TRPV1表达,雌激素可能通过nNOS和TRPV1调控下颌下腺唾液分泌功能。黑升麻和雌激素的作用机制并不相同,黑升麻不经nNOS和TRPV1发挥调控作用。
Objective To investigate the effects of estrogen and remifemin on the expression of neuronal nitric oxide synthase (nNOS), transient receptor potential vanilloid 1 (TRPV1), muscarinic acetylcholine receptor, member 1 and 3 (M1 and M3 receptor) and acetylcholinesterase (AChE) in the submandibular gland of rats. Methods Forty SD female adult rats were divided into SHAM group (sham operation), OVX group (ovarian removal), OVX+E group (ovarian removal + estrogen treatment) and OVX+ICR group (ovarian removal + remifemin treatment), 10 per group. The rats were recovered for 2 weeks after operation. The SHAM group and the OVX group were treated with distilled water, the OVX+E group and the OVX+ICR group were treated with β-estradiol and remifemin respectively. After 4 weeks, the location and expression of nNOS, TRPV1, M1 and M3 receptors in the submandibular gland were evaluated by immunohistochemistry. The changes of AChE expression in rat submandibular gland were observed by AChE staining. Results Compared with SHAM group (0.23±0.02, 0.28±0.01, 0.25±0.03, 0.19±0.03), the expression of nNOS, TRPV1, M1 and M3 receptors in OVX group (0.16±0.01, 0.21±0.01, 0.15±0.02, 0.09±0.02) were significantly lower (P<0.05);there were no significant difference between OVX+E group (0.23±0.01, 0.28±0.02, 0.23±0.03, 0.19±0.01) and SHAM group (P>0.05). But compared with OVX group, the expression of nNOS, TRPV1 and M3 receptors in OVX+ICR group were no significantly changed (P>0.05), and only M1 receptor expression (0.22±0.03) was significantly increased (P<0.05). The expression of AChE in OVX group (0.14±0.01) was significantly higher than that in SHAM group (0.10±0.01)(P<0.05). The expression of AChE in OVX+E group (0.15±0.01) was significantly higher than that in SHAM group (P<0.05). The expression of AChE in OVX+ICR group (0.09±0.01) was not significantly different from that in SHAM group (P>0.05). Conclusions Estrogen can significantly increase the expression of nNOS and TRPV1 in the submandibular gland of rats, suggesting that estrogen may regulate the salivary secretion function of the submandibular gland through nNOS and TRPV1. The mechanism of remifemin is different from that of estrogen, and remifemin does not play a regulatory role by nNOS and TRPV1.
作者
白芸
秦丽华
张义
姜海
王珂
贾静
Bai Yun;Qin Lihua;Zhang Yi;Jiang Hai;Wang Ke;Jia Jing(Shanxi Medical University School and Hospital of Stomatology, Taiyuan 030001, China;Depart merit of Anatomy and Histoembryology, School of Basic Medical Science, Peking University, Beijing 100191, China;Department of Stomatology, The Third Medical Center, Chinese PLA General Hospital, Beijing 100039, China)
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2019年第3期188-193,共6页
Chinese Journal of Stomatology
基金
国家自然科学基金(81570996、81571399).
关键词
颌下腺
雌激素类
升麻属
一氧化氮合酶Ⅰ型
Submandibular gland
Estrogens
Cimicifuga
Nitric oxide synthase type Ⅰ
