摘要
为评价水生蔬菜的抗氧化活性,采用超声破碎提取制备8种水生蔬菜的水提取物,研究提取物与ABTS^+自由基反应的紫外可见吸收光谱特征。结果表明,8种水生蔬菜提取物对ABTS^+自由基734 nm处吸收峰值随水生蔬菜用量增加呈剂量效应关系,相对于DPPH自由基清除试验基于517 nm处的吸收峰测量,ABTS^+自由基734 nm处吸收峰测量避免了水生蔬菜色素的光谱干扰,且ABTS^+自由基水溶性特点更适于日常饮食的抗氧剂摄入评价。8种水生蔬菜提取物的半数抑制浓度依次为:茭瓜(4.111 mg/mL)>慈姑(2.932 mg/mL)>荸荠(2.517 mg/mL)>海菜(2.413 mg/mL)>水芹菜(1.795 mg/mL)>水芋(1.594 mg/mL)>莲藕(0.974 mg/mL)>水蕨菜(0.140 mg/mL),水蕨菜具有最强的自由基清除活性。
Water extracts from 8 kinds of aquatic vegetables were prepared by ultrasonic crushing extraction to evaluate its antioxidant activity.The spectral characteristics of ABTS+free radical reacting with the extracts were investigated.It was found that the peak absorption of ABTS+free radical at 734 nm of 8 kinds of aquatic vegetable extracts showed a dose-effect relationship with the increase the adding of aquatic vegetable dosage after reacting.Compared with the DPPH free radical scavenging test,which was based on the absorption peak measure at 517 nm,the determination of absorption peak of the ABTS+free radical at 734 nm could avoid the spectral interference caused by aquatic vegetable pigments,moreover,the water-soluble characteristics of ABTS+free radicals were more suitable for the evaluation of antioxidant intake in the daily diet.The results showed that the half inhibitory concentrations of 8 aquatic vegetable extracts were as follows:Zizania Latifolia(4.111 mg/mL)>Sagittaria(2.932 mg/mL>Eleocharis Dulcis(2.517 mg/mL)>Edible Seaweed(2.413 mg/mL)>Oenanthe decumbens(1.795 mg/mL)>Calliopsis(1.594 mg/mL)>Nelumbo Nucifera Geartn(0.974 mg/mL)>Callipteris Esculenta(0.140 mg/mL).This showed that free radical scavenging activity of Callipteris Esculenta was the strongest.
作者
张海芬
高云涛
那吉
马娇
熊华斌
ZHANG Hai-fen;GAO Yun-tao;NA Ji;MA Jiao;XIONG Hua-bin(College of Chemistry and Environment,Yunnan Minzu University,Kunming 650500,Yunnan,China;Kunming Medical University Haiyuan College,Kunming 650106,Yunnan,China;West Yunnan University of Applied Sciences,Dali 617200,Yunnan,China)
出处
《食品研究与开发》
CAS
北大核心
2019年第6期8-13,18,共7页
Food Research and Development
基金
国家自然科学基金(21367025)
云南省科技计划项目(2017FD118)
云南民族大学研究生创新基金项目(2018YJCXS271)
关键词
抗氧化
ABTS^+自由基
水生蔬菜
光谱干扰
清除活性
antioxidant
ABTS^+free radical
aquatic vegetables
spectral interference
scavenging activity
