摘要
本研究用YC2×YF1抗、感组合的F2群体在两个环境中对蓖麻枯萎病抗性进行了QTL定位。遗传分析表明,群体中枯萎病抗性呈连续性偏态分布,后代表型偏抗性亲本。2014年检测到5个QTL,解释了总变异的18. 21%,单位点贡献率为0. 05%～12. 32%。2015年分别在出苗后20d、30d、40d、现蕾期和乳熟期进行了动态定位,分别检测出4、12、13、4和1个QTL,单位点贡献率为1. 64%～21. 91%,5个发育时期检出的QTL主要集中在第3、4和8连锁群上。有1个QTL在4个时期、3个QTL在3个时期、6个QTL在2个时期被重复检测到。在第8连锁群上一个QTL在两个环境同一时期被重复检测到。以上结果可为蓖麻抗枯萎病分子标记辅助选择提供参考。
In this study,the QTLs conferring the resistance to Fusarium wilt were mapped with the F2 populations derived from YC2 × YF1 resistant and susceptible combination under 2 environments in castor. Genetic analysis presented a continuous skewness distribution in populations,tending to resistant parent. 5 QTLs were identified in 2014,explained 18. 21% of phenotype variation,with a single locus contribution rate of 0. 05%-12. 32%.Dynamic mapping was performed at 5 stages including 20 d,30 d,40 d after seedling,squaring stage and milk ripening period in 2015,and 4,12,13,4 and 1 QTLs were detected respectively,with a single locus contribution rate of 1. 64%-21. 91%. 1,3 and 6 QTLs were identified simultaneously at 4,3 and 2 stages respectively. The detected QTLs at these 5 stages were mainly concentrated on linkage group 3,4 and 8. One QTL located on linkage group 8 was detected simultaneously in both environments at the same stage. The above results could facilitate the molecular marker assisted selection of Fusarium wilt resistance in castor.
作者
陈森
陆建农
施玉珍
汪亚菲
王亚如
何展泳
殷学贵
CHEN Sen;LU Jian-nong;SHI Yu-zhen;WANG Ya-fei;WANG Ya-ru;HE Zhan-yong;YIN Xue-gu(Guangdong Ocean University,Zhanjiang 524088,China)
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2019年第1期18-24,共7页
Chinese Journal of Oil Crop Sciences
基金
国家自然科学基金(31271759)
广东省科技计划项目(2013B060400024
2014A020208116
2016A020208015)
广东海洋大学创新强校工程项目(GDOU2013050206)
关键词
蓖麻
枯萎病
抗性
动态QTL定位
Ricinus communis L.
Fusarium wilt
resistance
dynamic QTL mapping
