摘要
Rapeseed,the largest oil crop in China,is a boron-loving plant.Boron has an important influence on the growth and development of rapeseed.In this study,four different boron-efficient Brassica napus varieties were used as the material to analyze the structure and sequence of BnNIP7;1 gene,aiming to provide a basis for studying the role of BnNIP7;1 in the absorption and transport of boric acid in rapeseed.The results showed that BnNIP7;1 exists in multiple copies of the Brassica napus genome,consisting of five exons and four introns.There are base insertions,base deletions and more base substitutions in introns.However,there is no base insertion or base deletion in the exon,only a small number of base substitution mutations are present,bringing about three amino acid changes in the encoded protein of BnNIP7;1-ZS9b in Zhongshuang 9.The BnNIP7;1-HY7b of Huayou 7 has an ochre mutation that will lead to a protein of only 47 amino acids,thus losing its function.Bioinformatics indicates that BnNIP7;1 protein is a membrane protein with six transmembrane regions,indicating that it is involved in the absorption and transport of boric acid.
油菜是我国第一大油料作物,也是一种喜硼植物,硼对其生长发育有重要的影响。研究以4种不同硼效率甘蓝型油菜(Brassica napus)品种(花油7号、中双6号、中双8号和中双9号)为材料,对BnNIP7;1基因的结构和序列进行分析。研究结果表明:BnNIP7;1在甘蓝型油菜基因组中多拷贝存在,由5个外显子和4个内含子组成,内含子中存在碱基插入、碱基缺失以及较多的碱基替换,但外显子中不存在碱基插入、碱基缺失,仅存在少量碱基替换突变,导致中双9号的BnNIP7;1-ZS9b基因编码蛋白有3个氨基酸改变,花油7号的BnNIP7;1-HY7b基因出现赭石密码突变而使得其编码蛋白仅47个氨基酸,丧失了功能。生物信息学表明BnNIP7;1蛋白是一种膜蛋白,具有6个跨膜区,说明它与硼酸的吸收转运有关。
基金
Supported by "System Construction of Rapeseed Industry" by Yunnan Agriculture Department(2017KJTX005-05)
"Yunnan Oil Crop Innovative Team" of Yunnan Science and Technology Department(2017HC021)~~
