摘要
采用盆栽试验,设置了不施氮肥(CK)、常规施肥(N2)、减施氮肥+纳米碳粉(N1+C)和常规施肥+纳米碳粉(N2+C)4个处理,研究了纳米碳配施氮肥对东北黑土养分和微生物群落结构的影响。结果表明,添加了纳米碳的处理土壤pH值和含水量均显著高于未添加纳米碳的处理,较N2处理土壤pH值平均高2.89%、土壤含水量平均高16.99%;添加纳米碳的处理土壤有机碳、速效磷和速效钾含量均显著高于未添加纳米碳的处理,分别高8.49%、7.49%和23.41%,且养分含量均以N1+C处理最高;土壤总PLFA含量表现为N2+C>N1+C>N2>CK,革兰氏阳性菌/阴性菌和细菌摩尔百分比表现为N1+C>N2+C>N2>CK,真菌和放线菌摩尔百分比表现为CK>N2>N1+C>N2+C。对土壤微生物群落结构进行主成分分析,结果表明,添加纳米碳使土壤微生物群落结构发生了改变,其中N2+C处理的影响最显著。可见,在农业生产中,纳米碳与氮肥配施对提高土壤养分含量、改善土壤微生物群落结构具有积极作用。
The effects of nanocarbon mixing with nitrogen fertilizer on microbial community structure and nutrient content of soil were studied, based on a pot experiment with four treatments: no nitrogen fertilizer(CK), conventional fertilizer(N2), decreased-nitrogen fertilizer plus nanocarbon(N1+C) and conventional fertilizer plus nanocarbon(N2+C). The results showed that compared with N2 treatment, the pH value and water content of soil of the treatments adding nanocarbon were significantly increased by 2.89% and 16.99% respectively. The SOC, Olsen P, available K were significantly increased by 8.49%, 7.49%, 23.41% respectively. The PLFA content was in the order of N2+C > N1+C > N2 > CK. The GNB to GPB ratio and the percentage of bacterial moles were in the order of N1+C > N2+C > N2 > CK. The percentage of fungi and the percentage of actinomyces were in the order of CK > N2 > N1+C > N2+C. The principal analysis(PCA)results showed that adding nanocarbon obviously changed the microbial community structure, and N2+C was the most obviously changed treatment. In conclusion, nanocarbon mixing with nitrogen fertilizer could increase the nutrient and water content, improve the microbial biomass and structure of soil.
作者
李一丹
孙磊
LI Yidan;SUN Lei(Institute of Soil Fertilizer and Environment Resources, Heilongjiang Academy of Agriculturual Sciences, Haerbin 150086, China)
出处
《中国稻米》
2019年第1期70-73,79,共5页
China Rice
基金
NAM长效专用肥料试验研究与示范(YS15B23)
黑龙江省农业科技创新工程2014年度院级科研项目计划(2014QN023)
