摘要
【目的】克隆马氏珠母贝(Pinctada martensii)法尼酸甲基转移酶(FAMe T)基因,并分析其在各组织中的表达。【方法】利用cDNA末端快速扩增技术(RACE)克隆获得马氏珠母贝FAMeT(PmFAMeT)基因的cDNA全长序列,利用实时荧光定量PCR(qPCR)方法分析PmFAMeT在马氏珠母贝不同组织中的表达模式。【结果与结论】PmFAMeT包含5′非编码区120 bp,3′非编码区968 bp和开放阅读框(ORF)1 536 bp,编码511个氨基酸。序列分析表明,PmFAMeT含有信号肽序列和跨膜结构域,并有WSC结构域、TSP1结构域和2个Methyltransf_FA结构域。将推导的PmFAMeT氨基酸序列与其他物种的FAMeT序列进行比对发现,不同物种的Methyltransf_FA序列同源性较高。PmFAMeT在马氏珠母贝的各个组织中均有表达,且在边缘膜、肝胰腺和性腺中的表达量显著高于其他组织。
【Objective】To clone the Farnesoic acid O-methyltransferase gene(PmFAMeT)and analyze its expression patterns in tissues of Pinctada martensii.【Method】The full-length cDNA sequence of PmFAMeT was cloned by rapid amplification of cDNA ends(RACE)and the expression of this gene was analyzed by RT-PCR.【Result and Conclusion】The PmFAMeT full-length cDNA contained 5′UTR(120 bp),the 3′UTR(968 bp)and open reading frame(1 536 bp)which encoded 511 amino acids.Analysis of deduced amino acids showed that it contained a signal peptide and transmembrane domain,WSC domain,TSP1domain and 2 Methyltransf_FA domain.Results from multi-sequence comparisons showed that the Methyltransf_FA domain of PmFAMeT has high homology compared with other species.PmFAMeT gene was expressed in the hepatopancreas,gonad,adductor muscle,marginal zone,pallial zone and central zone of P.martensii with higher expression level in the marginal zone,gonad and hepatopancreas(P<0.05).
作者
王姿曼
郝瑞娟
邓岳文
李俊辉
WANG Zi-man;HAO Rui-juan;DENG Yue-wen;LI Jun-hui(Fisheries College of Guangdong Ocean University,Zhanjiang 524088,China;Pearl Breeding and Processing Engineering Technology Research Center of Guangdong Province,Zhanjiang 524088,China)
出处
《广东海洋大学学报》
CAS
2019年第1期14-21,共8页
Journal of Guangdong Ocean University
基金
农业产业体系专项(CARS-49)
广东省自然科学基金项目(2017A030307024)
广东省省级科技计划项目(2017A030303076)
广东省渔港建设与渔业发展专项(B201601-Z09)
关键词
马氏珠母贝
法尼酸甲基转移酶
基因克隆
表达模式
Pinctada martensii
farnesoic acid O-methyltransferase
gene cloning
expression pattern
