摘要
目的调查新疆哈萨克族人群23个常染色体STR基因座遗传多态性,探讨其群体遗传关系及法医学应用价值。方法用华夏TM白金试剂盒,对新疆地区550名哈萨克族无关个体DNA进行扩增,3500XL遗传分析仪电泳分析,Gene Mapper ID-X v1. 4软件分析等位基因片段大小。统计分析23个STR基因座的频率数据和法医遗传学参数,并与其他地区已有人群数据进行比较。结果新疆哈萨克族各基因座个体识别率DP值在0. 820 6~0. 987 9,多态信息含量PIC值在0. 588 8~0. 920 0。累积个体识别率(CDP)和累积非父排除率(CPE)分别为1~9. 294 2×10-29和1~6. 9503×10-11。本研究根据Nei's DA遗传距离计算,新疆哈萨克族与数据库中的新疆和田哈萨克族的遗传距离最近(0. 004 4),与西藏藏族的遗传距离相对最远(0. 033 3)。结论这23个STR基因座在新疆哈萨克族人群中具有丰富的遗传多态性。研究不同民族群体的遗传多态性对了解他们的起源、迁移以及相互关系有重要的意义。
Objective To investigate the genetic polymorphisms of 23 autosomal short tandem repeats(STR)loci in Xinjiang Kazakh population,and to explore the population-based genetic relationships and to evaluate its potential application in forensic medicine.Methods The DNA from 550 unrelated individuals in Xinjiang Kazakh population were amplified using Huaxia^TM Platinum kit,and the PCR products were analyzed by electrophoresis through 3500XL genetic analyzer.The fragment size of alleles was analyzed by GeneMapper ID-X v1.4.Allele frequencies and forensic genetic parameters of 23 STR loci were statistically analyzed and compared with the available data of other populations from different races and regions.Results The power of discrimination(DP),and polymorphic information content(PIC) ranged from 0.8206 to 0.9879,0.5888 to 0.9200.The total discrimination power and cumulate probability of exclusion were 1-9.2942×10^-29 and 1-6.950 3×10^-11,respectively.Comparing with 23 reference populations,the genetic distance (Nei’s DA) between Xinjiang Kazakh in this study and Xinjiang Hotan Kazakh in data base in data base was the smallest(0.0044),while it was relatively larger between Xinjiang Kazakh and Tibet Tibetan (0.0333).Conclusions The 23 STR provided highly polymorphic information and forensic efficiency for forensic individual identification and paternity testing.The study of genetic diversity among different populations may support the research of their origins,migrations and their relationships.
作者
刘亚举
李效阳
郭利红
李学博
石美森
LIU Ya-ju;LI Xiao-yang;GUO Li-hong;LI Xue-bo;SHI Mei-sen(Xuchang Criminal Science and Technology Institute in Henan Province,Xuchang 461000;Henan Criminal Science and Technology Institute,Zhengzhou 450003;Key Laboratory of Evidence Identification in Universities of Shandong Province,Shandong University of Political Science and Law,Jinan 250014;Key Laboratory of Evidence Science of Ministry of Education,China University of Political Science and Law,Beijing 100192,China)
出处
《基础医学与临床》
CSCD
2019年第2期157-164,共8页
Basic and Clinical Medicine
基金
国家自然科学基金(81671874)
中国博士后科学基金(20170612712)
山东省自然科学基金(ZR2014HQ018)
