摘要
目的:建立狼疮合剂的质量控制标准。方法:对狼疮合剂中淫羊藿、鸡血藤、黄芪、白术进行薄层色谱法定性鉴别,对君药秦艽含有的龙胆苦苷进行高效液相色谱法含量测定。结果:薄层色谱图特征斑点清晰、分离较好,阴性对照无干扰;龙胆苦苷在0. 202 5～3. 24μg范围内与峰面积积分值呈良好的线性关系,平均加样回收率为102. 56%,RSD=1. 57%(n=6),3批狼疮合剂中龙胆苦苷平均含量为1. 70 mg/m L。结论:本法准确、灵敏,重复性好,为狼疮合剂的质量控制提供了依据。
Objective:To establish quality control standards of lupus mixture.Methods:Herba Epimedii,Caulis Spatholobi,Radix Astragali seu Hedysari,Rhizoma Atractylodis Macrocephalae of lupus mixture were identified by TLC (Thin-Layer Chromatography).Gentiopicroside of lupus mixture was determined by HPLC (High Performance Liquid Chromatography).Results:Characteristics of TLC spots were clear with better separation,and negative control had no interference.It was linear in the range of 0.2025-3.24 μg,and the rate of average recovery was 102.56% with RSD of 1.57%(n=6) for Gentiopicroside.Average concentrations of Gentiopicroside in three batches of Lupus mixture were 1.70 mg/mL.Conclusion:The method is accurate,delicacy and reproducible,providing a basis for quality control of lupus mixture.
作者
车晓平
王天园
王宏
韩旭阳
彭冰
曾祖平
Che Xiaoping;Wang Tianyuan;Wang Hong;Han Xuyang;Peng Bing;Zeng Zuping(Department of Pharmacy,Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,Beijing 100010,China;Department of Materia Medica,Beijing Institute of Traditional Chinese Medicine,Beijing 100010,China)
出处
《世界中医药》
CAS
2019年第1期73-76,共4页
World Chinese Medicine
关键词
狼疮合剂
龙胆苦苷
高效液相色谱法
薄层色谱法
Lupus mixture
Gentiopicroside
High performance liquid chromatography
Thin-layer chromatography
