摘要
目的探索志贺菌中成簇的规律间隔短回文重复序列(clustered regularly interspaced short palindromic repeats,CRISPR)与毒力基因和耐药基因的关系。方法从GenBank数据库中获得志贺菌的基因组、毒力基因和耐药基因的序列,通过多序列比对等方法获得志贺菌中CRISPR1、CRISPR2和CRISPR-Q1的分布情况、重复序列和间隔序列信息,通过BLAST方法获得毒力基因和耐药基因在志贺菌中的分布情况,SPSS 21.0软件分析CRISPR与毒力基因和耐药基因的关系。结果志贺菌中的CRISPR1和CRISPR2在不同菌株中的间隔序列差别较大,CRISPRQ1分布更为广泛(超过99%)。毒力基因在志贺菌中分布比较广泛(均超过56%),耐药基因中的blaOXA-1、camr、Sul2、tetB分布也比较广(超过50%)。CRISPR1/CRISPR2与是否携带9种毒力基因、4种耐药基因有关(P<0.05),CRISPR-Q1与是否携带3种毒力基因、4种耐药基因有关(P<0.05)。CRISPR1/CRISPR2和CRISPR-Q1均与志贺菌中存在的毒力基因和耐药基因的个数间有统计学关联(P<0.05)。CRISPR1/CRISPR2与CRISPR-Q1的间隔序列数目之间存在统计学关联(χ2=61.6,P<0.001)。结论志贺菌中不同CRISPR的分布差异比较大,存在的间隔序列数目不等;志贺菌CRISPR与部分毒力基因和耐药基因之间存在负相关;CRISPR1/CRISPR2与CRISPR-Q1的间隔序列数目存在正相关。
Objective To investigate the relationship of the clustered regularly interspaced short palindromic repeats(CRISPR)with resistance genes and virulence genes.Methods The Shigella genome sequences,resistance genes and virulence genes sequences were obtained from GenBank Database.Then we obtained the distribution of CRISPR1,CRISPR2 and CRISPR-Q1 and the information of repeat and spacers in Shigella by multiple sequences alignment.Meanwhile,the distribution of resistance genes and virulence genes in Shigella were obtained by BLAST and the relationship of the CRISPR with resistance genes and virulence genes was computed by SPSS 17.0.Results The spacers of CRISPR1 and CRISPR2 showed obvious differences in different Shigella strains,and the CRISPR-Q1 was more widely distributed(over 99%).The virulence genes were widely distributed in Shigella(more than 56%);the distribution of resistance genes(blaOXA-1,camr,Sul2,and tetB)was also wide(over 50%).There were significant differences between the CRISPR1/CRISPR2 and 9 kinds of virulence genes and 4 kinds of resistance genes(P<0.05).There were significant differences between the CRISPR-Q1 and 3 kinds of virulence genes and 4 kinds of resistance genes(P<0.05).There were significant differences between the CRISPR1/CRISPR2,CRISPR-Q1 and the number of virulence genes and resistance genes(P<0.05).There were significant differences between the CRISPR1/CRISPR2 and the number of spacers of CRISPR-Q1(χ^2=61.6,P<0.001).Conclusion The distribution of CRISPR was quite different in Shigella,and the number of spacers was also different.There were negative correlation of CRISPR of Shigella with some resistance genes and virulence genes.The CRISPR1/CRISPR2 had a positive relationship with the number of the spacers.
作者
王颖芳
王鹏飞
詹煜慧
张晓萌
段广才
郗园林
WANG Ying-fang;WANG Peng-fei;ZHAN Yu-hui;ZHANG Xiao-meng;DUAN Guang-cai;XI Yuan-lin(Department of Public Health,Medical College of Henan University of Science and Technology,Luoyang 471023;Hospital of Henan University of Science and Technology,Luoyang 471023;Medical College,Henan University of Science and Technology,Luoyang 471023;Department of Epidemiology,College of Public Health of Zhengzhou University,Zhengzhou 450001,China)
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2019年第1期71-75,共5页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
国家科技重大专项资助项目(No.2013ZX10004607
No.2018ZX10301407)~~
关键词
志贺菌
成簇的规律间隔短回文重复序列
毒力基因
耐药基因
Shigella
clustered regularly interspaced short palindromic repeats
resistance gene
virulence gene
