摘要
【目的】调查链格孢菌在菊科植物上的危害。【方法】2016—2017年,从湖北、北京、安徽及云南各地采集得到菊科千里光族植物瓜叶菊(Pericallis hybrida)、银叶菊(Senecio cineraria)及白子菜(Gynura divaricate)叶斑病病样4份。采用单孢分离的方法从各寄主上分别获得形态相似的链格孢菌大孢子种菌株。随后从各寄主分离物中筛选出1个代表菌株进行致病性、形态学及多基因位点(r DNA-ITS、GAPDH、Alt a 1、EF1-α、RPB2和ATPase)序列分析研究。【结果】4个菌株均可引起其寄主发生病害,且均为瓜叶菊链格孢菌(Alternaria cinerariae)。【结论】这是该菌种在国内的首次报道,也是该菌引起银叶菊与白子菜叶斑病病害的首次报道。
[Purpose]In order to investigate the damage caused by Alternaria species on Compositae plants.[Method]During2016-2017,4samples from3plants of Pericallis hybrida,Senecio cineraria,Gynura divaricate showing leaf spot or blight symptoms were collected from Hubei,Beijing,Anhui and Yunnan Province,China.Morphological similar large-spored Alternaria strains were obtained by single spore isolation from each sample.Then one representative strain from each host was selected to determine the pathogenicity,morphology and sequence analysis using six regions(rDNA-ITS,GAPDH,Alt a1,EF1-α,RPB2 and ATPase).[Result]The 4 strains were all causal pathogens on their hosts and identified as Alternaria cinerariae.[Conclusion]This is a new record of Alternaria species in China and it is also the first report causing leaf spot disease on S.cineraria and G.divaricate.
作者
罗欢
贾国庚
宋正兴
张宝玉
周燚
邓建新
LUO Huan;JIA Guogeng;SONG Zhengxing;ZHANG Baoyu;ZHOU Yi;DENG Jianxin(College of Agriculture,Yangtze University,Jingzhou 434025,China)
出处
《云南农业大学学报（自然科学版）》
CSCD
北大核心
2018年第6期1046-1053,共8页
Journal of Yunnan Agricultural University:Natural Science
基金
国家自然科学基金项目(31400014)
长江大学青年人才基金项目(2016cqr08)
