摘要
目的探讨Yes相关蛋白1(YAP1)在胆囊癌组织中的表达及其临床意义。方法选取62例胆囊癌及16例癌旁石蜡组织标本,常规福尔马林固定、脱水、包埋、切片处理,采用Envision二步法免疫组织化学染色法检测YAP1的表达情况,并分析癌组织YAP1阳性率与临床病理特征及预后的相关性。结果 YAP1在胆囊癌及癌旁组织阳性着色主要定位于细胞质和(或)细胞核,胆囊癌组织YAP1表达阳性率高于癌旁组织(P <0. 05)。胆囊癌癌组织YAP1表达阳性率在伴淋巴结或远处转移者中升高,且随原发肿瘤-区域淋巴结-远处转移(TNM)分期、分化程度的增加而增加,且胆囊癌YAP1表达阳性者预后较YAP1表达阴性者差(均P <0. 05)。结论胆囊癌癌组织中YAP1多呈阳性表达,且YAP1表达可能与胆囊癌TNM分期、分化程度、淋巴结转移及预后相关。
Objective To investigate the expression and its clinical significance of Yes-associated protein 1( YAP1) in gallbladder carcinoma tissues. Methods A total of 62 cases of gallbladder carcinoma and 16 paraffin tissue specimens were treated with formalin for conventional fixation,dehydration,embedding and tissue biopsies. The expression of YAP1 was detected by the Envision two-step immunohistochemical staining,and the correlation of the positive rate of YAP1 in carcinoma tissues with clinicopathological features and prognosis was analyzed. Results The positive staining of YAP1 was mainly localized in cytoplasm and/or nucleus of gallbladder carcinoma tissues and adjacent tissues,and the positive rate of YAP1 expression in gallbladder carcinoma tissues was higher than that in adjacent tissues( P < 0. 05). The positive rate of YAP1 expression in gallbladder carcinoma tissues increased in the cases complicated with lymphatic or distant metastasis,and increased with the increasing tumor-node-metastasis( TNM) stage and degree of differentiation,and the gallbladder carcinoma patients with positive expression of YAP1 had poorer prognosis compared with those with negative expression of YAP1( all P < 0. 05). Conclusion The expression of YAP1 in gallbladder carcinoma tissues is mostly positive,and the expression of YAP1 might correlate with TNM stage,degree of differentiation,lymphatic metastasis and prognosis of gallbladder carcinoma.
作者
黄晓峰
张翔
宋举贤
王宇笛
唐波
HUANG Xiao-feng;ZHANG Xiang;SONG Ju-xian;WANG Yu-di;TANG Bo(The Second Department of Surgery,the 925th Hospital of Joint Logistics Support Force,the Chinese People's Liberation Army,Guiyang 550009,China)
出处
《广西医学》
CAS
2018年第23期2764-2766,共3页
Guangxi Medical Journal
基金
贵州省自然科学基金(2017A030313688)
关键词
胆囊癌
癌组织
Yes相关蛋白1
病理
预后
Gallbladder carcinoma
Carcinoma tissue
Yes-associated protein 1
Pathology
Prognosis
