摘要
目的:建立一种快速、准确的超高效液相色谱串联质谱法(UPLC-MS/MS),用于临床上测定人血清中氯氮平、N-去甲基氯氮平、喹硫平、阿立哌唑及齐拉西酮5种常用的非典型抗精神病药物的血药浓度。方法:以氯氮平-d3、喹硫平-d8、阿立哌唑-d8和齐拉西酮-d8稳定同位素为内标,血清样品经过前处理后采用UPLCMS/MS对上清液进行分析。结果:空白血清对药物的检测无干扰,氯氮平、N-去甲基氯氮平、喹硫平、阿立哌唑及齐拉西酮在1. 00～1 000μg/L范围内线性良好,相关系数r> 0. 998 8;日内、日间精密度(RSD)均小于15%,回收率90%～110%;样品在室温12 h、4℃冰箱48 h、自动进样器24 h及反复冻融3次的情况下都能保持稳定。结论:本方法前处理操作简单,能够快速、准确、高通量地检测所选择的5种非典型抗精神病药物的血药浓度。
Objective:To establish a fast and precise method for simultaneously determining the five atypical antipsychotics clozapine,N-desmethyl clozapine,quetiapine,aripiprazole and ziprasidone in human serum by ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).Methods:Clozapine-d3,quetiapine-d8,aripiprazole-d8 and ziprasidone-d8 were used as internal standards and the serum samples were analyzed by UPLC-MS/MS after pretreatment.Results:The detection result of the five drugs was not disturbed by blank matrix;the linearity of five drugs was well in the range of1.00~1000μg/L and the correlation coefficients were higher than0.9988;the relative standard deviations(RSDs)of intra-and inter-day precision were lower than15%;the average recoveries were between90%to110%.All the five drugs remained stability under the condition of room temperature for 12hours,4degrees centigrade in fridge for48hours,automatic sampler for24hours and after three freeze-thaw cycles.Conclusion:The pretreatment process is very simple and this method can meet the requirements of fast,accuracy and high throughput in clinical in monitoring this five atypical antipsychotics in clinical.
作者
王玮
余淑珍
彭军
许洪涛
WANG Wei;YU Shuzhen;PENG Jun;XU Hongtao(Department of Pharmacy, Jiangsu Integrated Traditional Chinese and Western Medicine Hospital, Nanjing 210028,Jiangsu, China;Hangzhou BIOZON Medical Institute Co., Ltd, Hangzhou 310030, Zhejiang, China)
出处
《贵州医科大学学报》
CAS
2018年第12期1425-1429,共5页
Journal of Guizhou Medical University
关键词
超高效液相串联质谱
血药浓度
非典型抗精神病药物
血清
同位素内标
ultra-high performance liquid chromatography tandem mass spectrometry
blood drug concentration
atypical antipsychotics
serum
isotope internal standard
