摘要
为规范水稻细菌性条斑病抗性的鉴定方法,分别采用喷湿法、剪叶法、针刺法和压渗法对10份水稻材料进行抗性鉴定。结果表明,喷湿法可以准确地区分水稻材料的抗感差异,抗性材料发病较轻或不发病,而易感病材料发病严重,但病斑位置分布不均匀,无法通过测量病斑面积而量化分级;剪叶法发病较轻或不发病,无法区分水稻材料的抗感差异;针刺法和压渗法都可以准确地区分出水稻材料的抗感差异,且对10份水稻材料的抗性级别鉴定结果基本一致,但总体上针刺法测量病斑的变异系数要大于压渗法,且对于同一水稻材料采用针刺法所测的病斑长度要小于压渗法。
To standardize the resistance identification method for rice bacterial leaf streak(BLS)disease,four different inoculation methods,namely,spray method,leaf cutting method,acupuncture method,and infiltration method,were compared on10rice acessions.The results showed that the spray method could accurately distinguish the rice accessions for the resistance trait.The resistant accessions had mild or no disease incidence,but the susceptible accessions were severely infected.However,disease rating could not be done due to the uneven distribution of lesions and inability to measure the area of the lesions quantitatively.The leaf cutting method produced mild or no disease lesions and could not distinguish the resistant accessions from the others.Both acupuncture and infiltration methods could accurately distinguish the difference among the10rice accessions for disease resistance or susceptibility with the same results,although the coefficients of variation by the acupuncture method were generally larger than those by the infiltration method.In addition,the length of lesions from the acupuncture method was smaller than that from the infiltration method involving the same rice accession.
作者
赵严
罗登杰
何圣贤
刘芳
ZHAO Yan;LUO Dengjie;HE Shengxian;LIU Fang(College of Agriculture,Guangxi University/State Key Laboratory for Protection and Utilization of Subtropical Agro-bioresources,Nanning,Guangxi 530004,China)
出处
《亚热带农业研究》
2018年第4期242-246,共5页
Subtropical Agriculture Research
基金
国家自然科学基金(31460341)
广西科技计划(桂AB16380093)
广西自然科学基金(2013GXNSFAA019060)资助项目
关键词
水稻
细菌性条斑病
抗性鉴定
接种方法
rice
bacterial leaf streak
resistance identification
inoculation method
