摘要
目的:检测中药木鳖子乙醇提取物对羟基桂皮醛(CMSP)的毒性,为其应用于临床肿瘤治疗提供毒理学依据。方法:60只昆明小鼠(4～6周龄,雌雄各半)分为空白对照组,CMSP200mg/kg和400mg/kg染毒组,每组20只,采用腹腔注射的方式染毒,每周3次,每次100μL,连续28d。观察各组小鼠一般状态,28d后,处死小鼠,摘眼球取血,采用全自动血细胞分析仪及全自动生化分析仪检测小鼠血常规、肝肾功能和心肌酶谱等生化指标,并计数雄性小鼠精子畸形率,同时采用HE染色观察各组小鼠重要脏器组织的病理改变。观察骨髓细胞微核形成率。结果:观察期内无小鼠死亡,各组小鼠血常规指标、雄性小鼠精子畸形率与对照组相比,差异均无统计学意义(P>0.05),高剂量CMSP组小鼠外周血ALT和AST较对照组高(P<0.05),肾功能及心肌酶谱无明显差异(P>0.05)。HE染色结果显示各组小鼠内脏组织均未观察到肉眼可见的病理改变。小鼠骨髓细胞微核形成率亦无明显变化(P>0.05)。结论:在本研究中,我们发现CMSP具有相对较小的毒性作用,因此可考虑应用于肿瘤临床治疗中,但在后续研究中需充分关注其可能具有的肝功能损伤作用。
OBJECTIVE:This experiment mainly studied the toxicological effect of p-hydroxylcinnamaldehyde(CMSP)extracted from the Cochinchina momordica seeds(CMS),on mice and provided a basis for clinical use of this drug.METHODS:60 Kunming mice(4-6 weeks old)were randomly divided into three groups:control,200 mg/kg treatment and 400 mg/kg treatment groups.CMSP was administered by intraperitoneal injection.The general behavior,routine blood test,clinical biochemical test and teratospermia of the mice were evaluated.Furthermore,the pathologic changes of the vital organs were detected by immunohistochemical assay.Finally,the micronucleus formation in bone marrow cells was determined.RESULTS:There were no observed abnormal activities,blood test and teratospermia in CMSP treated-mice during the periods.However,the higher ALT and AST concentrations in serum were observed in the mice treated with 400 mg/kg CMSP.There were no abnormal changes of renal function,myocardial enzyme spectrum and pathologic changes of the vital organs.Moreover,there was no increase of micronucleus rate in bone marrow cells of treated mice.CONCLUSION:In our experiment,CMSP may have little toxic and side effects,so it may be considered for use in clinical treatments.However,it is also necessary to fully consider its possible toxicity to liver functions.
作者
马鸣
白函瑜
李晓亚
王延海
赵日旸
戴素丽
武一鹏
张璁
赵连梅
单保恩
MA Ming;BAI Hanyu;LI Xiaoya;WANG Yanhai;ZHAO Riyang;DAI Suli;WU Yipeng;ZHANG Cong;ZHAO Lianmei;SHAN Baoen(Clinical Laboratory of the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011;Research Centre of the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011;Hebei Military Outpatient Department,Shijiazhuang 050011,Hebei,China)
出处
《癌变·畸变·突变》
CAS
CSCD
2018年第6期452-456,462,共6页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金(81703073)
河北省自然科学基金(H2018206115)
关键词
木鳖子
对羟基桂皮醛
毒理作用
肿瘤
临床治疗
Cochinchina momordica
p-hydroxylcinnamaldehyde
toxicity effect
tumor
clinical treatment
