摘要
从药用植物地黄中分离出一株产红色素内生真菌RJL03(Monascus sanguineus),为进一步提高菌株RJL03的红色素产量,采用响应面法(RSM)对其液态摇瓶发酵条件进行研究。在单因素实验的基础上,以发酵液OD值为指标,利用Box-Behnken中心组合实验设计原理(BBD)优化菌株液态发酵产红色素的工艺条件。结果显示,该色素发酵液在474 nm处有最大吸收波长,最佳发酵工艺为温度30℃、装瓶量67%、初始p H自然(7)。在此条件下液态发酵菌株,显著提高了菌株RJL03的红色素产量。
A strain of red pigment fungi RJL03(Monascus sanguineus)was isolated from the medicinal plant Rehmannia glutinosa Libosch by tissue block separation methd.Monascus sanguineu s was identified according to morphological characteristics and rDNA ITS1-5.8S-ITS2 sequence analysis.Response Surface Methodology(RSM)was used to further improve the red pigment yield of RJL03 strain.On the basic of single factor tests,the strain was fermentation process of the liquid shake flask by using the Box-Behnken design(BBD),with the optical density of the fermentation broth as an index.The experiment manifests that the fermentation broth of pigment has its maximal absorption wavelength at 474 nm.The optimum fermentation conditions were as follows:temperature 30℃,bottling volume 67%,initial pH natural(7).After the fermentation of the strain,optimization by RSM significantly increased the yield of the target strain.
作者
何亚涛
刘俊林
郑青波
柳玉瑛
罗琨映
高丹丹
HE Ya-tao;LIU Jun-lin;ZHENG Qing-bo;LIU Yu-ying;LUO Kun-Ying;GAO Dan-dan(College of life science and Engineering,Northwest Minzu University,Lanzhou 730124,China;School of Chemical Engineering,Northwest Minzu University,Lanzhou 730124,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2018年第3期438-443,共6页
Natural Product Research and Development
基金
西北民族大学中央高校基本科研业务费(Z17001
31920170178)
西北民族大学"双一流引导专项"生物工程特色学科建设(10018704)
甘肃省科技计划(1504WKCA094)
科技部援助项目(KY201501005)
