摘要
【目的】明确猪圆环病毒3型(Porcine circovirustype 3,PCV3)在广西猪群中的致病性及流行病学特点,为有效防控其暴发流行提供参考依据。【方法】采用PCR对广西某猪场腹泻死亡仔猪的病料样品进行PCV3检测,并对其唯一结构蛋白质(Cap)的结构进行同源模拟,构建遗传进化树;同时在线(http://www.cbs.dtu.dk/services/)预测Cap蛋白信号肽、糖基化位点和B细胞抗原表位。【结果】在广西某猪场腹泻死亡仔猪的病料样品中检测到PCV3。PCR扩增产物经核苷酸序列测定分析后截取Cap基因序列(645 bp),命名为PCV3/CN/Guangxi001/2017。PCV3/CN/Guangxi001/2017与13株国内PCV3毒株和5株美国PCV3毒株的Cap基因序列同源性分别在96.9%~99.4%和98.3%~99.5%,属于3b亚群;而与PCV1毒株和PCV2毒株的核苷酸序列同源性只有43.0%和45.8%,且B细胞抗原表位差异明显,在PCV1特有的B细胞抗原表位(92~103 aa)、PCV2特有的B细胞抗原表位(69~83 aa、117~131 aa和231~233 aa)及PCV1、PCV2共有的B细胞抗原表位(156~162 aa和179~192 aa)均无相似性。【结论】广西猪群中已存在PCV3感染,鉴于PCV2与PCV3间无交叉免疫保护特性,实际生产中应通过加强清洁消毒、灭鼠杀虫、定期监测、自繁自养等生物安全措施防控PCV3暴发流行。
【Objective】The present study explored the pathogenicity and epidemiology characters of porcine circovirus type3(PCV3)in Guangxi in order to provide the references for prevention and control of PCV3.【Method】PCV3were detected by PCR from epidemic materials of piglets died from diarrhoea.Homology modeling was conducted on its only structural protein(Cap),and phylogenetic tree was constructed.Signal peptide,glycosylation site and cell B epitope of Cap protein of PCV3were redicteded on-line(http://www.cbs.dtu.dk/services/).【Result】Epidemic materials of piglets died from diarrhoea was positive for PCV3.Its PCR amplification products was analyzed by nucleotide sequence,then gene sequence of Cap was intercepted(645bp)and named PCV3/CN/Guangxi001/2017.PCV3/CN/Guangxi001/2017shared96.9%-99.4%and98.3%-99.5%homology with Cap gene sequence of13PCV3strains isolated from China and5PCV3strains isolated from American respectively.However,its nucleotide sequence homology to PCV1and PCV2were only43%and45.8%,and differences in cell B epitope was obvious.Cell B epitope(92-103aa)peculiar to PCV1,cell B epitope(69-83aa,117-131aa and231-233aa)peculiar to PCV and shared cell B epitope(156-162aa and179-192aa)in PCV1and PCV2had no similarity.【Conclusion】PCV3has been emerging in pig herd in Guangxi.Since there is no cross immunity between PCV2and PCV3,biosafety control measures including sterilization,insecticides,rodenticides,regular monitoring and self-supportive should be performed in pig farm to prevent outbreak of PCV3.
作者
贺会利
李军
潘艳
胡帅
冯世文
彭昊
李常挺
陈泽祥
杨威
HE Hui-li;LI Jun;PAN Yan;HU Shuai;FENG Shi-wen;PENG Hao;LI Chang-ting;CHEN Ze-xiang;YANG Wei(Guangxi Veterinary Research Institute/Guangxi Key Laboratory of Veterinary Biotechnology,Nanning 530001,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2017年第8期1499-1503,共5页
Journal of Southern Agriculture
基金
广西科学研究与技术开发计划项目(桂科重14121003-3-1)
广西水产畜牧兽医局科技项目(桂渔牧科201633002)
