摘要
通过批试实验研究了同步亚硝化、厌氧氨氧化和反硝化(SNAD)生物膜的脱氮性能.SNAD生物膜具有良好的厌氧氨氧化和反硝化活性.厌氧氨氧化NH_(4)^(+)-N、NCV-N和总无机氮(TIN)去除速率分别为0.121,0.180,0.267kgN/(kgVSS·d);反硝化和亚硝态氮氧化活性分别为0.211,0.053kg NO_(2)^(-)-N/(kg VSS·d).SNAD生物膜厌氧氨氧化适宜的pH值范围为5~9,生物膜有助于缓解pH值对厌氧氨氧化菌的抑制作用.SNAD生物膜对NO_(2)^(-)-N和FNA的抑制作用表现出良好的耐受能力.当NO_(2)^(-)-N浓度分别为100,150mg/L时,对应的FNA浓度分别为70,100ng/L,厌氧氨氧化NH_(4)^(+)-N去除速率分别为0.087,0.029kg N/(kg VSS·d).扫描电镜显示,在SNAD生物膜表面主要是一些短杆菌.在SNAD生物膜内部主要为火山口状细菌,应为厌氧氨氧化菌.
The nitrogen removal performance of simultaneous partial nitrification,anaerobic ammonium oxidization and denitrification(SNAD)biofilm was investigated in batch tests.The SNAD biofilm performed good anammox and denitrification activities.The NH_(4)^(+)-N,NO_(2)^(-)-N and total inorganic nitrogen(TIN)removal rates of anammox activity were0.121,0.180and0.267kg N/(kg VSS·d),respectively.The denitrification and nitrite oxidation activities were0.211and0.053kg NO_(2)^(-)-N/(kg VSS·d),respectively.The suitable pH ranges for anammox activity were5.0to9.0.The the inhibition effect of pH on the anammox bacteria could be largely relieved by biofilm.Besides,the SNAD biofilm was found to perform high tolerance to the inhibition of NO_(2)^(-)-N and free nitrous acid(FNA).The NH_(4)^(+)-N removal rates for anammox process were0.087and0.029kg N/(kg VSS·d)with the NO_(2)^(-)-N concentrations of100mg/L(FNA concentration of70μg/L)and150mg/L(FNA concentration of100μg/L),respectively.The SEM observations indicated that the bacteria in the outer part of the SNAD biofilm were mainly short rod-shaped.In the inner part of the SNAD biofilm,the bacteria were mainly crater-shaped,which should be anammox bacteria.
作者
郑照明
李军
侯爱月
马静
杜佳
赵白航
杨京月
ZHENG Zhao-ming;LI Jun;HOU Ai-yue;MA Jing;DU Jia;ZHAO Bai-hang;YANG Jing-yue(National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology,Engineering Research Center of Beijing,Beijing University of Technology,Beijing 100124,China)
出处
《中国环境科学》
EI
CAS
CSSCI
CSCD
北大核心
2017年第4期1322-1330,共9页
China Environmental Science
基金
国家水体污染控制与治理科技重大专项(2014ZX 07201-011)
国家自然科学基金青年基金(51308010)
教育部博士点新教师(20131103120017)
北京市博士后工作经费资助项目(2015ZZ-10)